马尾藻多糖对鸡脾脏淋巴细胞GSH和GSSG水平的影响  

Effect of Sargassum Polysaccharide on Levels of GSH and GSSG of Splenic Lymphocytes in Chickens

作　　者：黎江[1] 尹丹[1] 刘洪丽[1] 何颖[2] 胡庭俊[1] 

机构地区：[1]广西大学动物科学技术学院,广西南宁530005 [2]广西壮族自治区兽医研究所,广西南宁530001

出　　处：《动物医学进展》2015年第4期46-49,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31260619)

摘　　要：为观察马尾藻多糖(SP)对鸡脾脏淋巴细胞内氧化还原状态的影响,无菌分离鸡脾脏淋巴细胞,采用终浓度为12.5、25、50、100、200μg/mL的马尾藻多糖分别刺激培养鸡脾脏淋巴细胞4、8、12、24h,测定细胞内还原型谷胱甘肽(GSH)和氧化型谷胱甘肽(GSSG)水平。结果表明,各浓度SP与鸡脾脏淋巴细胞共同培养4、8、12h均能不同程度地升高细胞内GSH水平,降低鸡脾脏淋巴细胞内GSSG水平,升高鸡脾脏淋巴细胞内GSH/GSSG比值,与空白对照组相比差异显著或极显著(P<0.05或P<0.01)。说明SP可以通过调节鸡脾脏淋巴细胞内GSH和GSSG的水平来调节细胞内的氧化还原状态,从而发挥其抗氧化作用。The study was carried out to investigate effect of Sargassumpolysaccharide（SP）on redox state of splenic lymphocytes in chickens.Splenic lymphocytes were isolated aseptically,SP at concentration of12.5,25,50,100,200μg/mL was used in splenic lymphocytes for 4,8,12,24 hto observe the levels of reduced glutathione sodjum（GSH）and glutathione oxidized（GSSG）.The results showed that SP at concentration of 12.5,25,50,100,200μg/mL significantly increased the level of GSH,decreased the level of GSSG and increased the ratio of GSH/GSSG compared with the blank control（P0.05 or P0.01）.From the findings,it could be concluded that SP markedly enhance antioxidant activity by regulating the redox state of splenic lymphocytes in chickens.

关 键 词：马尾藻多糖 还原型谷胱甘肽 氧化型谷胱甘肽 抗氧化 

分 类 号：S852.163[农业科学—基础兽医学] S859.7[农业科学—兽医学]