蒙古沙冬青AmDREB2C基因的克隆及表达分析  

Cloning and Expression Analysis of Am DREB2C in Ammopiptanthus mongolicus

作　　者：郭婷[1] 王茅雁[1] 董博[1] 姜晓旭[1] 武雅琪 

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018

出　　处：《植物遗传资源学报》2015年第2期344-348,共5页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(31260256);内蒙古自然科学基金(2012ZD02)

摘　　要：蒙古沙冬青(Ammopiptanthus mongolicus)是中国西北荒漠区唯一的常绿阔叶灌木,具有很强的抗寒抗旱特性。利用PCR方法从该植物克隆到转录因子Am DREB2C的c DNA和基因组DNA的全长编码区,二者均由1191 bp组成,无内含子序列,编码由396个氨基酸残基组成的蛋白,其中含1个AP2结构域和1个核定位信号。表达分析显示,Am DREB2C的转录受低温和干旱胁迫的诱导。此外,将该基因编码区c DNA成功构建到植物表达载体p CAMBIA3300-35ST上,为后续研究其功能奠定了基础。Ammopiptanthus mongolicus,the only evergreen broad-leaf shrub in the northwest desert of China,shows very strong resistance to cold and drought stresses. The complete coding region c DNA and genomic DNA fragments of the transcription factor Am DREB2 C were cloned by PCR approach from A. mongolicus. Both the fragments contained 1191 base pairs,therefore Am DREB2 C was intronless. The predicted Am DREB2 C protein was composed of 396 amino acid residues,with a conserved AP2 domain and a nuclear localization signal. Expression analysis revealed that the transcription of Am DREB2 C was induced by cold and drought stresses. Moreover,the c DNA fragment of Am DREB2 C was successfully inserted into the plant expression vector p CAMBIA3300-35 ST,laying a foundation for further functional analysis.

关 键 词：蒙古沙冬青 DREB 基因克隆 表达分析 

分 类 号：S687.2[农业科学—观赏园艺]