大豆类钙调磷酸酶B亚基GmCBL1互作候选蛋白的筛选  

Screening of Candidate Interacting Proteins of the Calcineurin B-like Protein GmC BL1 in Soybean

作　　者：李林蔚[1,2] 张双喜[3] 周永斌[2] 裴丽丽[2] 陈明[2] 李连城[2] 马有志[2] 刘生祥[1] 徐兆师[2] 

机构地区：[1]宁夏大学农学院,银川750021 [2]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/农业部麦类生物学与遗传育种重点实验室,北京100081 [3]宁夏农林科学院农作物研究所,永宁750105

出　　处：《植物遗传资源学报》2015年第2期359-363,共5页Journal of Plant Genetic Resources

基　　金：转基因生物新品种培育科技重大专项(2014ZX08002-002;2014ZX08003-004B)

摘　　要：Ca2+是非生物胁迫信号转导途径中的重要信号分子,植物类钙调磷酸酶B亚基蛋白(CBL,calcineurin B-like proteins)是一类重要的钙信号受体蛋白,主要通过与其他蛋白的特异结合传递信号,使植物形成对非生物胁迫的响应。本实验室已经获得大豆Gm CBL1基因,功能鉴定显示Gm CBL1增强了转基因拟南芥对非生物胁迫的耐性。为了进一步研究Gm CBL1的作用机理,本研究构建诱饵载体p GBKT7::Gm CBL1,利用酵母双杂交技术筛选大豆Gm CBL1的互作蛋白。通过对筛选获得的106个蛋白基因测序和Blast比对分析,并根据其可能的生理功能对这些候选蛋白归类,整理得到4类蛋白:能量代谢相关蛋白、修饰蛋白、防御蛋白、钙信号转导相关蛋白。筛选得到候选蛋白的功能预测初步表明,大豆Gm CBL1参与多条信号途径,为进一步研究探索大豆CBL介导的抗逆信号转导途径奠定了基础。Calcium is a critical messenger in abiotic stress responses signal transduction pathways in plants.Calcineurin B-like proteins（ CBLs） represent a family of plant calcium sensor proteins that function in calcium signaling by associating with their interacting protein to transduce signal,making plant response to abiotic stress. Our laboratory has identified a putative soybean CBL gene that functions in abiotic stress tolerance in the transgenic Arabidopsis plants. In order to further explore the Gm CBL1-mediated stress resistance mechanism,the mixture of constructed p GBKT7： ： Gm CBL1 bait vectors and c DNA library was introduced into yeast competent cells AH109 to screen for the interaction proteins from soybean c DNA library by yeast-two hybrid system. A total of 106 candidate positive clones were sequenced and analyzed through BLAST. According to their possible physiological functions,those candidate proteins were classified into four categories including energy metabolism proteins,modified protein,defense protein,and calcium signaling transduction proteins. Function prediction of the candidate proteins suggestedthat Gm CBL1 was possibly involved in several stress signal transduction pathways. This work would provide a basis for a defined further functional dissection of CBL-mediated signaling system in soybean.

关 键 词：大豆 类钙调磷酸酶B亚基 GmCBL1 酵母双杂交 互作蛋白 

分 类 号：S565.1[农业科学—作物学]