两个育性相关基因在BNS和YS小麦温敏雄性不育系中的表达差异分析  

Differential Expression Analysis of Two Male Fertility- related Genes in Wheat Thermo-sensitive Male Sterile Line BNS and YS( Triticum aestivum L. )

作　　者：张芳凝 李桂冬 范晓静[1] 王震[1] 张淼[1] 马翎健[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《植物遗传资源学报》2015年第2期368-372,共5页Journal of Plant Genetic Resources

基　　金：国家科技支撑计划(2011BAD35B03);西北农林科技大学基础科研业务费项目(ZD2012001)

摘　　要：为研究雄性不育相关基因TA1和TA2在BNS和YS小麦温敏雄性不育系732A花粉发育时期的表达特点,探讨这2个育性相关基因与温敏雄性不育小麦育性转换的联系,本研究利用荧光实时定量PCR方法,在BNS和YS型不育系732A花药发育四分体期、单核期、二核期和三核期定量检测基因TA1和TA2的mRNA表达水平。结果表明:(1)在732A和BNS花粉发育四分体时期至二核期,基因TA1相对表达量上调,在三核期相对表达量下降;(2)基因TA2相对表达量在BNS花粉发育的四分体时期至二核期逐渐下降,三核期上升;在732A花粉发育4个时期中的相对表达量变化刚好相反;(3)在BNS和732A花粉发育二核期,基因TA1和TA2均表现极值,推测二核期可能为BNS和YS型小麦温敏雄性不育系花粉发育最敏感时期;(4)在不育系BNS和732A花粉发育过程中,基因TA1的相对表达量变化幅度比TA2的高。推测TA1对不育系BNS和732A花粉败育影响程度强于TA2;(5)基因TA1和TA2相对表达量在BNS的花粉发育时期表达趋势相反,推测其对BNS花粉败育影响表现为拮抗作用,且2个基因不连锁;在732A花粉发育时期表达趋势相同,推测其对不育系732A花粉败育影响表现为协同作用。This study focus on the expression characteristics of wheat male sterility related gene TA1 and TA2 in the pollen development of BNS and YS-type wheat thermo-sensitive male sterile line 732 A. The expression differences may show the relationship between the two genes and the fertility transformation of wheat thermo-sensitive male sterile line. In this study,mRNA expression level of TA1 and TA2 were examined by fluorescence real-time quantitative PCR（ qRT-PCR）,taking anthers of male sterile line BNS and 732 A as materials,and anthers were collected at four key developmental stages（ tetrad phase,uninucleated period,binucleated period and trinucleate period） respectively. The results showed that ：（ 1） The relative expression of TA1 increased from tetrad phase to binucleated period and decreased in trinucleate period of pollen development.（ 2） The relative expression of TA2 decreased from tetrad phase to binucleated period and increased since trinucleate period in BNS,while the relative expression showed the opposite changes in 732 A.（ 3） The relative expression of TA1 and TA2 both showed extreme value in bi-nucleated period of pollen development,demonstrating the key stage of pollen abortion might be″binucleated period″for sterile line BNS and 732 A.（ 4） During the pollen development process of sterile lines BNS and 732 A,the variation range in relative expression of TA1 was higher than that of TA2. This indicated that TA1 might play a more influential role in pollen abortion of BNS and 732 A than TA2.（ 5） Gene TA1 and TA2 showed opposite effects in pollen development of sterile line BNS,manifesting that these two non-allelic genes played antergic roles in pollen abortion of BNS. In the contrast,these two genes expressed consistently in the pollen development of 732 A,showing that they worked synergisticly in the pollen abortion of 732 A.

关 键 词：小麦 TA1 TA2 BNS 732A 荧光实时定量PCR 

分 类 号：S512.1[农业科学—作物学]