益气化瘀解毒法对慢性萎缩性胃炎伴异型增生大鼠DNMT1的影响  

Effect of Yiqi Huayu Jiedu Principle on the DNMT1 in Chronic Atrophic Gastritis with Dysplasia in Rats

作　　者：贺梅娟[1] 杨晋翔[2] 安静[1] 赵晨[1] 刘婷[1] 

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学第三临床医学院,北京100029

出　　处：《中医学报》2015年第3期396-398,共3页Acta Chinese Medicine

基　　金：国家自然科学基金项目(81173232)

摘　　要：目的:观察益气化瘀解毒法对慢性萎缩性胃炎伴异型增生模型大鼠DNMT1蛋白的影响。方法:将Wistar大鼠60只随机分为造模组50只、空白组10只。造模成功后将造模后剩余的33只大鼠随机分为模型组11只,维酶素组11只,消痞颗粒组11只。空白组、模型组给予生理盐水,维酶素组给予维酶素悬浊液,消痞颗粒组给予消痞颗粒中药制备药液,1次·d-1。连续治疗12周后用Western blot法对各组大鼠DNMT1蛋白进行检测。结果:模型组DNMT1蛋白表达量(0.293±0.036)较空白组(0.133±0.025)显著升高(P<0.01);消痞颗粒组(0.173±0.044)较模型组(0.293±0.036)及维酶素组(0.229±0.032)降低(P<0.05);消痞颗粒组蛋白表达量(0.173±0.044)与空白组(0.133±0.025)比较,差异无统计学意义(P>0.05)。结论:中医益气化瘀解毒法可以逆转胃黏膜异型增生,这种作用可能是通过下调DNMT1而实现的。Objective ： To observe the effect of Yiqi Huayu Jiedu principle on the DNMT1 in chronic atrophic gastritis with dysplasia in rats. Methods：Wistar rats （n = 60） were randomly divided into modeling group （n = 50 ） and blank group （n = 10 ）. After successful modeling the rest of rats （n = 30） were randomly divided into model group ,Weimeisu group and Xiaopi granule group （each n = 10）. Themodel group was give normal saline water, Weimeisu group was given Weimeisu turbid liquid and Xiaopi granule group was given the Xiaopi granule （once a day）. After continuous treatment for 12 weeks, the method of Western blot was used for detecting DNMTI pro- tein in rats of all groups. Results：The expression of DNMT1 protein increased significantly in model group compared with blank group （ P 〈 0.01 ）, decreased in Xiaopi granule group compared with model group and Weimeisu group （ P 〈 0.01, P 〈 0.05 ）. The difference ih the comparison between Xiaopi granule group and blank group had no statistical significance （ P 〉 0.05 ）. Conclusion ： Chinese medicine Yiqi Huayu Jiedu can reverse the gastric mucosal dysplasia,this effect may be mediated by inhibit the expression of DNMTI.

关 键 词：慢性萎缩性胃炎伴异型增生 益气化瘀解毒法 DNMT1 消痞颗粒 大鼠 

分 类 号：R285.5[医药卫生—中药学]