心脑宁片对D-半乳糖致小鼠亚急性衰老模型胸腺组织的影响  

Effect of Xinnaoning Tablet on Thymus Tissue of D-galactose Induced Subacute Aging Model

作　　者：申晋昌[1] 王灵[1] 吕宏迪[1] 汤寅[1] 郝少君[1] 张正臣[1] 

机构地区：[1]中国人民解放军第371中心医院,河南新乡453000

出　　处：《中医学报》2015年第3期402-404,共3页Acta Chinese Medicine

基　　金：河南省科学技术厅科技发展计划项目(092102310202)

摘　　要：目的：观察心脑宁片对 Ｄ－半乳糖致小鼠亚急性衰老模型胸腺组织形态的影响。方法：取体质量１８～２１ｇ小鼠７２只，雌雄各半，其中 １２只为空白对照组，余 ６０只每天颈背部皮下注射 Ｄ半乳糖（生理盐水配成质量浓度为 ５％，注射量为 ０．０２５ｍＬ·ｇ^－１）制作糖代谢衰老模型，每天给药 １次，连续 ４０ｄ。模型制作成功后，随机平均分为模型组 （生理盐水０．０２ｍＬ·ｇ^－１），脑安片组（０．５３ｇ·ｋｇ^－１，２６．５ｇ·Ｌ^－１，０．０２ｍＬ·ｇ^－１，相当于临床用量的１５倍），大剂量、中剂量、小剂量心脑宁片组（２．２４ｇ·ｋｇ^－１、１．１２ｇ·ｋｇ^－１、０．５６ｇ·ｋｇ－１，１１２ｇ·Ｌ^－１、５６ｇ·Ｌ^－１、２８ｇ·Ｌ^－１，０．０２ｍＬ·ｇ^－１，相当于临床剂量的 ３０倍、１５倍、７．５倍）。每天给药１次，连续３０ｄ。取小鼠胸腺组织用福尔马林固定，石蜡包埋，切片，ＨＥ染色，光镜观察胸腺组织形态的变化。结果：模型组胸腺皮质厚度、淋巴细胞数分别为（７．２６±２．１８）μｍ、（２１．４±３．７）个，脑安片组分别为（２３．１８±９４５）μｍ、（３６．３±１２．２）个，大剂量心脑宁片组分别为（２７．８９±３．４６）μｍ、（４６．６±１０．４）个，中剂量心脑宁片组分别为（２４３６±４１９）μｍ、（３７．５±８．６０）个，小剂量心脑宁片组分别为（２２．１８±３．４５）μｍ、（３６．３±７．７）个，空白对照组分别为（２８６２±４３５）μｍ、（５７．３±１１．４）个。各组胸腺皮质厚度、淋巴细胞数与模型组比较，差异均有统计学意义（Ｐ＜０．０１）。结论：心脑宁片可显著增加 Ｄ半乳糖致小鼠亚急�Objective：To observe the effect of Xinnaoning Tablet on thymus tissue of D-galactose induced subacute aging model. Methods ：72 mice, male and female half, 12 for blank control group, the rest 60 mice were given back neck subcutaneous injection of D-galactose（ with physiological saline into mass concentration 5% , injection volume of 0. 025 mL · g^- 1 ） to form aging model, 1 times a day ,40 D in a row. After the success of the model making, randomly divided into model group （ physiological saline 0.02mL · g^- 1 ） , Naoan group （0.53 g · kg^-1,26.5g · kg^-1,0.02 mL · g^-1, 15 times of clinical dosage）,large dose group, middle dose and small dose of Xinshenning （2.24 g · kg^-1 ,1.12 g · kg^-1 ,0.56 g · kg^-1 ,112 g · kg^-1 ,56g · kg^-1 ,28 g · kg^-1,0.02 mL · g^- 1,equivalent to 30 times, 15 times ,7.5 times of clinical dosage） , once a day for 30 d. Thymus tissue in mice with formalin fixed, paraffin embedding, section, HE stain, light microscopy to observe the organization change. Results ： The thymus cortex thickness and lymphocyte count of modelgroup respectively was （7.26 ± 2.18 ） um and （21.4 ± 3.7 ）, in Naoan group respectively was （ 23.18 ± 9.45 ） um and （ 36.3 ± 12. 2 ） , in large dose group of Xinnaoning was respectively （ 27.89 ± 3.46 ）um and （ 46.6 ± 10.4 ） , in middle dose group was respectively （24.36 ± 4.19 ）um and （37.5 ± 8.60） ,in small dose group was respectively （22.18 ± 3.45 ） um and （36.3 ± 7.7 ） and in blank control group was respectively （ 28.62 ± 4.35 ） um and （ 57.3 ± 11.4 ）. Compared with model group, the difference had statistical significance （P 〈 0.01 ）. Conclusion：Xinnaoning Tablet can significantly increase the thymus tissue thickness and lymphoeytes number of D-galactose induced subacute aging model mice with good anti-aging effect.

关 键 词：心脑宁片 胸腺组织 D-半乳糖 亚急性衰老模型 脑安片 小鼠 

分 类 号：R285.5[医药卫生—中药学]