林蛙卵雌二醇的超临界CO_2萃取及酶联免疫分析  

Supercritical Fluid Extraction of Estradiol from Rana Chensinensis Egg and Analysis by ELISA

作　　者：邢燕[1] 邱芳萍[1] 纪可[1] 杜琳[1] 

机构地区：[1]长春工业大学,吉林长春130012

出　　处：《食品研究与开发》2015年第1期7-9,共3页Food Research and Development

基　　金：吉林省科技发展计划项目(20110252)

摘　　要：以长白山林蛙卵为原料,研究超临界CO2萃取林蛙卵中的雌二醇的工艺条件。探讨萃取压力、萃取温度、CO2流量和萃取时间对雌二醇萃取率的影响,并采用酶联免疫分析法检测林蛙卵雌二醇的含量。结果表明最佳萃取条件为萃取压力30 MPa,萃取温度47℃,CO2流量为12 kg/h,萃取时间为90 min。酶联免疫分析得到林蛙卵中雌二醇的含量为38.11 ng/L。The text used Rana chensinensis egg as material,effects of extraction pressure, temperature,flowrate and time on yield of estradiol extracted by supercritical carbon dioxide were studied , and the content of estradiol was detected by ELISA. The results showed that the optimal extraction conditions were follow ：extraction pressure 30 MPa ,temperature 47℃, carbon dioxide flowrate 12 kg/h and extraction time 90 min. The content of estradiol detected by ELISA was 38.11 ng/L.

关 键 词：超临界CO2萃取 林蛙卵 雌二醇 酶联免疫分析 

分 类 号：TQ461[化学工程—制药化工]