固相萃取-液相色谱测定葡萄干中防腐杀菌剂  

Determination of Preservatives and Bactericides in Raisins by Solid Phase Extraction-Liquid Chromatography

作　　者：史龙梅[1] 李欢[1] 周建科[1] 

机构地区：[1]河北大学理化分析中心,河北省分析科学技术重点实验室,河北保定071002

出　　处：《食品研究与开发》2015年第1期103-105,共3页Food Research and Development

摘　　要：以无水硫酸钠为分散剂,固相分散萃取葡萄干中噻苯咪唑、三唑醇、灭菌丹、咪酰胺4种防腐杀菌剂,C18固相萃取小柱净化处理,高效液相色谱法测定。反相C18色谱柱分离,乙腈-甲醇-水(50∶10∶40,体积比)为流动相,检测波长225 nm。在0.01μg/m L^200μg/m L范围内4组分线性关系良好,相关系数大于0.999 3,方法检出限为2.0 ng/g^20.0 ng/g,相对标准小于3.18%,方法适合于葡萄干中相关防腐杀菌剂的检测。With anhydrous sodium sulfate as dispersant , Four kinds of preservatives and bactericides in raisins were extracted by solid phase dispersion method , the C18 solid phase extraction small column was used for purification. Thiabendazole, Baytan, Folpet and Prochloraz were separated by reversed -phase high performance liquid chromatography with C18 column. The mobile phase was the mixture of acetonitrile , methanol and water （50 ∶ 10 ∶ 40,v/v） and detection wavelength was 225 nm. The linearity of regression equations were good in the range of 0.01 μg/mL-200 μg/mL, with correlation coefficients were greater than 0.999 3. Limit of detection is 2.0 ng/g-20.0 ng/g. The relative standard deviations （RSD） were less than 3.18 %. This method is suitable for the analysis of relevant preservatives and bactericides in raisins.

关 键 词：固相萃取 高效液相色谱 噻苯咪唑 三唑醇 灭菌丹 咪酰胺 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]