L-苯丙氨酸解氨酶产生菌的原生质体诱变育种  

Mutation Breeding of Protoplast of L-phenylalanine Ammonia-lyase Producing Strains

作　　者：李丽[1] 熊思驰[1] 黄飞 王国盼[1] 韦春葵[1] 苏宏飞[1] 梁智群[1] 黄时海[1] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530005 [2]南宁高新区人才交流中心,广西南宁530007

出　　处：《食品研究与开发》2015年第1期109-113,共5页Food Research and Development

基　　金：南宁市科学研究与技术开发计划项目(20125193);梧州市科学研究与技术开发计划项目(201201023)

摘　　要：原始菌株海洋红酵母M1202产生的L-苯丙氨酸解氨酶(PAL),能够逆反应催化反式肉桂酸(t-Ca)生成L-苯丙氨酸。对M1202进行原生质体紫外诱变获得一株转化率为原始菌株115%的菌株TM2,经6次传代,仍具有较好的遗传稳定性。Using Rhodotorula benthica M1202 as the original strain, the L-phenylalanine ammonia-lyase （PAL） of M1202 could transform trans-Cinnamic acid （t-Ca） to L-phenylalanine. The aim of this study was to obtain new Rhodotorula benthica mutants with high conversion rate. UV induced mutagenesis of protoplast was performed on original strain Rhodotorula benthica M1202. A genetically stable mutant strains TM2 was selected from a large amount of the regenerative mutants. The conversion rate of TM2 was 1.15-fold increased compared with its original strain. Further experiment confirmed after six generations successively propagating the conversion rate of TM2 was stable.

关 键 词：海洋红酵母 L-苯丙氨酸解氨酶 原生质体诱变 

分 类 号：Q933[生物学—微生物学]