黄酮类化合物对耐药金黄葡萄球菌mecA基因的影响  

The effects of flavonoids on mec A gene of Drug-resistant staphylococcus aureus

作　　者：刘立新[1] 高月林[2] 张羽男[1] 张楠楠[1] 

机构地区：[1]黑龙江省教育厅生物药制剂重点实验室,佳木斯大学药学院,黑龙江佳木斯154007 [2]黑龙江农业职业技术学院,黑龙江佳木斯154007

出　　处：《黑龙江医药科学》2015年第1期4-6,共3页Heilongjiang Medicine and Pharmacy

基　　金：黑龙江省教育厅科学技术研究项目;编号:12521560

摘　　要：目的:研究黄酮类化合物对人工诱导的耐药金黄葡萄球菌的耐药基因mec A的mRNA表达水平的影响。方法:采用纸片法测定黄酮类化合物对耐药金黄葡萄球菌的逆转作用;利用RT-PCR法测定耐药基因mec A的相对表达量。结果:耐药金黄葡萄球菌分别被芦丁、姜黄素、木犀草素、槲皮素和芹菜素作用后,耐药基因mec A的相对表达量分别为0.55,0.79,0.76,0.92,和0.84。结论:黄酮类化合物能降低金黄葡萄球菌的耐药基因mec A的表达,能对抗金黄葡萄球菌的耐药性。Objective： To study the effects of flavonoids on the expression levels of mecA of Drug - resistant staphylococcus aureus. Methods： The reversal effects of drug - resistant staphylococcus aureus were determined by K - B paper methods. The expression levels of meeA were determined by RT - PCR method. Results： Rutin, cur- cumin,luteolin, quercetin and celery were acted on staphylococcus aureus, respectively which showed that the ex- pression levels of mecA were 0. 55 , 0. 79 , 0. 76 ,0.92 and 0.84. Conclusion： Flavonoids can reduce the meca gene expression of drug - resistance Staphylococcus aureus, and it can compete against drug resistance of Staphylo- coccus aureus.

关 键 词：黄酮类化合物 金黄葡萄球菌 耐药基因 

分 类 号：R378.11[医药卫生—病原生物学]