佳木斯地区健康人群与高血压患者CYP3A4* 1G基因多态性分析  

The polymorphisms analysis of healthy people and hypertension patients with CYP3A4* 1G gene in Jiamusi

作　　者：左璐[1] 方芳[1] 杨军[1] 隋小芳[1] 张志国[1] 雷力力[1] 

机构地区：[1]佳木斯大学附属第一医院,黑龙江佳木斯154003

出　　处：《黑龙江医药科学》2015年第1期7-9,共3页Heilongjiang Medicine and Pharmacy

基　　金：黑龙江省自然科学基金项目;编号:2011-86;紫禁城国际药师论坛专题科研课题;编号:ZJC-2012-30

摘　　要：目的:通过比较本地区健康人与高血压患者CYP3A4*1G基因多态性的差异,探讨CYP3A4*1G基因多态性与高血压病的易感关系。方法 :采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法检测本地区健康人与高血压患者的CYP3A4*1G基因型分布及等位基因频率,通过χ2检验来比较本地区健康人与高血压患者基因型分布差异和等位基因频率差异。结果:高血压患者与健康人的基因分布及等位基因频率差异有显著性和高度显著性(P<0.05和P<0.01)。结论:高血压病的发生与CYP3A4*1G的碱基变异具有相关性。Objective： To explore the relationship between CYP3A4 ~ 1G gene polymorphisms and the Hyper- tension pathogenesis, by comparing the differences of CYP3A4 ＊ 1G Gene polymorphisms in patients with hyperten- sion and healthy populations in Jiamusi. Method： Polymerase chain reaction -restriction fragment length polymor- phism （PCR -RFLP） method was applied to detect the genotype distribution and the allele frequencies of CYP3A4 ＊ 1G in patients with hypertension and healthy people; and to compare the differences between them according to Chi -square criterion. Results： There existed significant differences in the gene distribution and allele frequencies of CYP3A4 ＊ 1G in patients with hypertension and healthy people （P 〈 0.05 ,P 〈 0.01 ）. Conclusion：The hyper- tension pathogenesis is related with the base variations of CYP3A4 ＊ 1G.

关 键 词：高血压 CYP3A4＊1G 基因多态性 PCR-RFLP 

分 类 号：R544.1[医药卫生—心血管疾病]