重酒石酸长春瑞滨长循环脂质体的制备与表征  

Preparation and Characterization of Vinorelbine Bitartrate Long-circulation Liposome

作　　者：谢向阳[1] 陈晨[1] 林雯[2] 李银科[1] 李旸[1] 陈鹰[1] 

机构地区：[1]广州军区武汉总医院药剂科,武汉430070 [2]湖北省黄石市爱康医院检验科,黄石435000

出　　处：《医药导报》2015年第3期379-384,共6页Herald of Medicine

摘　　要：目的采用p H梯度法制备重酒石酸长春瑞滨长循环脂质体并进行表征。方法以粒径为指标,考察水化温度和挤出次数对空白脂质体粒径的影响;以粒径及包封率为指标,考察孵化温度和孵化时间对载药脂质体粒径和包封率的影响。并采用Malvern粒度仪测定脂质体的粒径分布、多分散系数及Zeta电位,透射电镜考察其形态,并考察脂质体稳定性。结果重酒石酸长春瑞滨长循环脂质体粒径(96.4±27.2)nm,多分散系数(0.162±0.042),Zeta电位(-26.7±3.5)m V;透射电镜显示脂质体粒径均一,成单层膜球状分布;长期稳定性研究显示,脂质体在5℃条件下放置3个月稳定。结论 p H梯度法可以用于重酒石酸长春瑞滨长循环脂质体的制备。Objective To prepare vinorelbine bitartrate long-circulation liposomes by pH gradient loading methods and make characterization. Methods The impact of hydration temperature and extrusion times on the blank liposome particle size was investigated;and the incubation temperature and in duration on size and encapsulation percentage of drug loading liposome particle was tested. The vinorelbine bitartrate long-circulation liposome was characterized for particle size,polydispersion index, Zeta potential,morphology,and was studied for long term stability. Results The particle size,Zeta potential,polydispersion in-dex of long-circulation liposomes were （96. 4±27. 2） nm,（0. 162±0. 042）,（-26. 7±3. 5） mV,respectively. The liposomes were small,unilamellar and spherical with smooth surface under transmission electron microscopy. Long term stability studies showed that the liposomes were stable for up to 3 months after storage at 5 ℃ . Conclusion The preparation technology for the vinorel-bine bitartrate long-circulation liposome by pH gradient loading methods is feasible.

关 键 词：长春瑞滨 重酒石酸 脂质体 长循环 p H梯度法 

分 类 号：R979.1[医药卫生—药品] TQ450.1[医药卫生—药学]