五肽Pro-Gly-His-Glu-Tyr的合成及与铜(Ⅱ)离子的作用  

Synthesis of a pentapeptide Pro-Gly-His-Glu-Tyr and its interaction with copper(Ⅱ) ion

作　　者：王召璐 李磊[1] 李艳[1] 李连之[1] 

机构地区：[1]聊城大学化学化工学院,山东聊城252059

出　　处：《化学研究与应用》2015年第3期315-319,共5页Chemical Research and Application

基　　金：国家自然科学基金项目(21142003)资助

摘　　要：通过固相多肽合成法合成了一个新的五肽Pro-Gly-His-Glu-Tyr,电喷雾质谱法测得其分子量与理论值一致。利用荧光光谱法研究了该五肽与金属离子的相互作用。结果表明,Cu2+对该肽具有很强的荧光猝灭效应,而其他金属离子的荧光猝灭较弱。该肽对Cu2+的这种特异性作用是由其独特的氨基酸序列所决定的。荧光光谱研究表明,Cu2+对五肽的猝灭机理为静态猝灭,五肽与Cu2+形成复合物的结合常数Ka为5.54×104。A new pentapeptide Pro-Gly-His-Glu-Tyr was synthesized via Fmoc solid-phase peptide synthesis. The molecular weight determined by ESI-MS is consistent with its theoretical value. The interaction of the pentapeptide with metal ions was also investigated by fluorescence spectroscopy. Results showed that Cu^2＋had a high-efficiency fluorescence quenching,but other commonly metal ions only had a little quenching effects. This specific role towards to Cu^2＋was determined by the unique amino acid sequence of the pentapeptide. The fluorescence spectral results indicated that the possible quenching mechanism of the pentapeptide by Cu^2＋was a static quenching,and their association constant Kawas 5. 54×10^4.

关 键 词：多肽 金属离子 Cu2+离子 荧光光谱 

分 类 号：O629.72[理学—有机化学]