Fe_3O_4磁性纳米粒子催化动力学荧光法测定微量过氧化氢  

Kinetic spectrofluorimetric determination of hydrogen peroxide with Fe_3O_4 magnetic nanomaterials

作　　者：李俊[1] 李焱[1] 彭金娜[2] 冯素玲[2] 

机构地区：[1]焦作师范高等专科学校理工学院,河南焦作454000 [2]河南师范大学化学化工学院,河南新乡453007

出　　处：《化学研究与应用》2015年第3期388-392,共5页Chemical Research and Application

基　　金：河南省科技厅软科学项目(122400450221)资助;河南省教育厅高等学校青年骨干教师资助计划项目(2013GGJS-266)资助;焦作市应用基础研究计划项目(SJC20130004)资助

摘　　要：Fe3O4磁性纳米粒子（Fe3O4MNPs）具有模拟过氧化氢酶的催化特性。基于Fe3O4MNPs催化H2O2氧化吡罗红建立了催化动力学荧光法测定H2O2的方法。对Fe3O4MNPs-吡罗红-H2O2体系的荧光光谱进行了研究,并考查了一些因素对体系的影响。所研究的催化反应分别在7-13min和13-18min内为假零级反应,对应的表观速率常数分别为1.33×10^-1s^-1和8.41×10^-2s^-1。反应时间为18 min时,催化反应的表观活化能为40.42 kJ·mol^-1。在最佳实验条件下,方法的线性范围为3.20×10^-4-1.12×10^-2mol·L^-1,检出限为1.65×10^-4mol·L^-1。该法可用于消毒液、雨水、自来水及合成样品中H2O2含量的测定。Fe3O4magnetic nanomaterials exhibit an intrinsic peroxidase-like catalytic activity. A new kinetic spectrofluorimetric method was developed for the determination of H2O2 based on the catalytic activity of Fe3O4 MNPs for the oxidation of pyronine by H2O2. The characteristic spectra of fluorescence of the system were discussed. The effects of some experiments conditions were investigated. The catalytic reaction were considered as pseudozero order in 7 - 13 min and 13 - 18 min,respectively,and the corresponding apparent rate constants were 1. 33×10-1s-1and 8. 41×10-2s-1. When the reacting time was taken as 18 min,the apparent activity energy of the catalytic reaction was found to be 40. 42 k J·moL^-1. Under the optimum conditions,the linear range of H2O2 was 3. 20×10^-4- 1. 12 ×10^-2mol·L^-1with the limit of detection 1. 65×10^-4mol·L^-1. This method was suitable for the determination of hydrogen peroxide in disinfector,rain water,tap water and synthetic samples.

关 键 词：过氧化氢 Fe3O4MNPs 吡罗红 动力学荧光法 

分 类 号：O675.3[理学—化学]