高效液相法测定中药健胃愈疡片中延胡索乙素的含量  

Determination of Tetrahydropalmatine in Jianwei Ulcer Tablets by RPHPLC

作　　者：马荔 滕再进 王志强 戴青 

机构地区：[1]南京圣和药业股份有限公司,南京210038

出　　处：《海峡药学》2015年第2期45-47,共3页Strait Pharmaceutical Journal

摘　　要：目的建立高效液相方法测定健胃愈疡片中延胡索乙素的含量。方法色谱柱:Luna C18(5μm,250×4.6mm)柱;流动相:0.1%磷酸溶液(三乙胺调p H至6.0)-甲醇(45∶55)为流动相;流速为1.0m L·min-1;检测波长为280nm。结果平均回收率为96.44%,RSD为0.70%。结论本方法测定延胡索乙素的含量精密度好,准确性高,可作为健胃愈疡片的质量控制指标。OBJECTIVE To develop a HPLC method for quantitative determination of Tetrahydropalmatine in Jianwei ulcer tablets.METHODS HPLC was performed on a luna C18 （4.6mm ×250mm,5μm） column;With mo-bilephase of 0.1%H3PO4（adjust to pH 6.0 with Triethylamine）-MeOH（45∶55）;The flow rate was 1.0mL· min -1;The detective wavelength was 280nm.RESULTS The average recovery for Tetrahydropalmatine was 96.44%with a RSD of 0.70%.CONCLUSION The method is simple,accurate and can be used to control the quality of Jianwei ulcer tablets.

关 键 词：健胃愈疡片 高效液相色谱法 延胡索乙素 

分 类 号：R927.2[医药卫生—药学] R282.71