DNA甲基化检测技术进展及经验总结  

Progresses and Experiences of DNA Methylation Detection Techniques

作　　者：李懿[1] 李光润[1] 陈宏[1] 

机构地区：[1]成都军区昆明总医院肿瘤科,昆明650032

出　　处：《医学综述》2015年第2期204-207,共4页Medical Recapitulate

基　　金：国家自然科学基金(81201756);云南省自然科学基金(2012FD091)

摘　　要：DNA甲基化作为一种表观遗传学元件,参与正常细胞的发育分化、基因组印记、X染色体失活和染色质重构等生命过程,在肿瘤演进中亦发挥重要作用。为满足不断深入的研究需求,DNA甲基化检测技术得到了较快的发展,检测的灵敏度和特异度不断提高,并逐步从个别位点向高通量检测发展。该文综述了目前常用的DNA甲基化分析方法,并简要总结了其在应用中的关键点和经验。DNA methylation,as an epigenetic component,is involved in the processes of cell development,differentiation,genomic imprinting,X chromosome inactivation and chromatin remodeling,and plays an important role in carcinogenesis as well. The DNA methylation analysis technology has been developing rapidly due to the needs of deeper research： analyses that previously were restricted to specific loci can now be performed on a high throughput,meanwhile the sensitivity and specificity have made great of improvements as well. Here is to make a review of the methods to detect DNA methylation and summarize the key points and experiences in application.

关 键 词：表观遗传学 DNA甲基化 检测 

分 类 号：Q3-3[生物学—遗传学] Q751