毛细管电泳法分离4种手性药物对映体  

Chiral separation of 4 drugs by capillary electrophoresis

作　　者：孙嘉仪 邢玉平[1] 宋佳新 郭兴杰[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2015年第3期199-203,共5页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(81273476)

摘　　要：目的建立以羧甲基-β-环糊精(carboxymethyl-β-cyclodextrin,CM-β-CD)为手性选择剂的毛细管电泳法拆分氯苯那敏、羟氯喹、班布特罗和沙丁胺醇4种手性药物。方法采用未涂层石英毛细管柱,背景电解质为Na H2PO4缓冲溶液,分离电压为20 k V。考察了CM-β-CD的质量浓度、缓冲溶液质量浓度和p H值对拆分的影响。结果在最佳分离条件下,氯苯那敏、羟氯喹、班布特罗和沙丁胺醇的分离度分别为10.49、6.97、3.75和1.56。结论 CM-β-CD对研究的4种药物有非常高的对映体选择性。Objective To present a study on enantiomeric separation of chlorphenamine, hydroxychloroquine, bambuterol and salbutamol by using CM-β-CD as chiral selector. Methods The separations of the enantiomers were performed in an untreated fused-silica capillary tube in NaH2PO4 buffer with a voltage of 20 kV. The effects of pH value, chiral selector concentration and concentration of buffer on the enantiomeric separation of the selected drugs were investigated and optimized. Results Under the optimal conditions, the resolution of hydroxychloroquine, chlorphenamine ,bambuterol and salbutamol were 10.49,6.97,3.75 and 1.56, respectively. Conclusions CM-β-CD is highly enantioselective for the 4 basic drugs.

关 键 词：毛细管电泳法 羧甲基-β-环糊精 对映体分离 

分 类 号：R917[医药卫生—药物分析学]