不同强度超声波辅助浸浴法导入草鱼嗜水气单胞菌疫苗免疫效果评价  

Immunity Evaluation of Aeromonas hydrophila Vaccine Bathed Accompanying Treatment with Different Ultrasound Intensity in Grass Carp Ctenopharyngodon idellus

作　　者：蒋昕彧[1] 张超[1] 赵燕静[1] 李旭东[2] 王飞[2] 孔祥会[1] 

机构地区：[1]河南师范大学水产学院,河南新乡453007 [2]河南省水产技术推广站,河南郑州450008

出　　处：《水产科学》2015年第3期155-160,共6页Fisheries Science

基　　金：河南省科技厅成果转化项目(132201610019);河南省水产学重点学科资助项目(2012)

摘　　要：采用不同强度的超声波处理,研究鱼类免疫效应和疫苗导入效果,从而优化最适超声波处理强度,以便应用于渔业实践中。本研究中,在56kHz的超声频率下,采用不同超声强度（0、83、166、250、333mW/cm2）,用以辅助浸浴法导入草鱼嗜水气单胞菌疫苗。结果显示,用166、250mW/cm2的超声波强度,免疫14d后,超声处理组溶菌酶活性和白细胞吞噬活性最高,显著高于对照组（P〈0.05）;免疫28d后,血清凝集效价达到最高,显著高于对照组（P〈0.05）。超声波强度为166、250mW/cm2时,超声波处理组的相对免疫保护率高于对照组和其他处理组,分别达到66.5%和83.5%。本研究表明,用166mW/cm2和250mW/cm2的超声波强度处理后,鱼体免疫效价显著提高,表明该强度可作为适宜的超声波处理强度。实际生产中可采用166~250mW/cm2超声波强度处理,以辅助浸浴法有效导入草鱼细菌性疫苗,研究结果对于渔业生产应用和示范推广将提供重要指导。The transcutaneous immunisation of a vaccine Aeromonas hydrophila was studied in grass carp Ctenopharyngodon idellus under different ultrasound intensities（0,83,166,250,and 333 mW/cm2）at ultrasound frequency of 56 kHz.The maximal lysozyme and phagocytic activities were observed under ultrasound intensities of 166mW/cm2 and 250mW/cm2 on day 14 after vaccination,significantly higher than those in the control group（P〈0.05）.The maximal agglutinating antibody titers were found in the immune challenged groups on day 28 after vaccination,significantly higher than those in the control group（P〈0.05）.There was higher immunity protection at 166mW/cm2 and 250mW/cm2 than in the control,indicating that these ultrasound intensities can be used to treat the fish,combining with the vaccine bathing,to promote the vaccine into the fish and to increase the immunity effect of the vaccine.

关 键 词：超声波 浸泡疫苗 嗜水气单胞菌 草鱼 

分 类 号：S965.112[农业科学—水产养殖]