胸腺五肽对脓毒症大鼠肾损伤保护作用的实验研究  

Influence of thymopentin-5 on renal pathology and relevant indexes of serum in rats with acute kidney injury caused by sepsis

作　　者：朱永蒙[1,2] 王凌峰[3] 郭卫东[4] 

机构地区：[1]Collage of Life Science of Inner Mongolia University [2]Department of Urinary Surgery, Baogang Hospital of Inner Mongolia [3]Department of Burn, Baogang Hospital of Inner Mongolia [4]Department of Oncology, Baogang Hospital of Inner Mongolia

出　　处：《中华临床医师杂志（电子版）》2015年第5期80-83,共4页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的观察胸腺五肽(TP-5)对脓毒症大鼠肾损伤的影响。方法采用脓毒症大鼠模型,选用雄性SD大鼠90只,按随机数字表法随机分为3组:对照组,脓毒症组,胸腺五肽处理组。于造模腹壁缝合后1 h、6 h、12 h、24 h和48 h分别测定各组血清中尿素氮(Bun)和肌苷(Cr)浓度;光镜下观察肾组织损伤情况;再分别测定各组血清中肿瘤坏死因子α(TNF-α)、白细胞介素-10(IL-10)浓度和CD4+/CD8+的变化。结果脓毒症组和胸腺五肽处理组大鼠6 h及以后时点Bun、Cr的浓度明显高于对照组(P<0.05);胸腺五肽处理组6 h、12 h、24 h、48 h与脓毒症组比较Bun、Cr明显降低(P<0.05)。与对照组比较,从1 h时点开始脓毒症组和胸腺五肽处理组大鼠TNF-α、IL-10浓度开始升高,并且脓毒症组和胸腺五肽处理组TNF-α、IL-10浓度在各时点均明显高于对照组(P<0.05);胸腺五肽处理组与脓毒症组比较,各时点TNF-α、IL-10的浓度明显降低(P<0.05)。脓毒症组和胸腺五肽处理组大鼠CD4+/CD8+比值从6 h开始下降,24 h达最低,48 h升高,且明显低于对照组(P<0.05);胸腺五肽处理组1 h以后各时点与脓毒症组比较CD4+/CD8+比值明显升高(P<0.05)。结论胸腺五肽可抑制脓毒症大鼠肾损伤。ObjectiveTo discuss thymopentin-5 on renal pathology and relevant indexes of serum with sepsis-caused acute kidney injury （AKI） caused by cecal ligation and puncture （CLP）. Methods90 cases of healthy and male SD rats were randomly divided into 3 groups： control group （C group）, sepsis group （S group）, thymopentin group （T group）. These groups were divided into five time point including 1 h, 6 h, 12 h, 24 h and 48 h with 6 rats in each time point. The sepsis model was made by CLP. The blood and kidney tissue were collected in each timepoint. Changes of renal pathology were observed under light microscope and relevant indexes like serum creatinin （Cr）, blood urea nitrogen （Bun）, tumor necrosis factorα （TNF-α）, interleukin-10 （IL-10）, CD4＋/CD8＋ was tested and analyzed.Results In T group, concentrations of Cr, Bun in 6 h after CLP starts to rise, reached peak in 24 h and decreased in 48 h, which were all lower than S group （P〈0.05） and higer than C group （P〈0.05）. Compared with C group, concentrations of TNF-α in 1 h significantly rise, reached peak in 12 h and decreased in 24 h,which were all lower than S group （P〈0.05） and concentrations of IL-10 in 1 h significantly decreased, reached peak in 12 h and rise in 24 h,which were all more than S group （P〈0.05）. In T group, CD4＋/CD8＋ ratio in 6 h after CLP starts to decreased reached the lowest in 24 h and rise in 48 h, which were all lower than S group （P〈0.05） and significantly lower than C group （P〈0.05）.Conclusion Thymopentin-5 plays the role of renal protection in AKI caused by sepsis.

关 键 词：胸腺五肽 脓毒症 肾损伤 

分 类 号：R459.7[医药卫生—急诊医学]