4种罗非鱼不同组织中HSP70基因对盐胁迫的响应  

The HSP70 gene expression response of four strains of tilapia to salt stress in different tissues

作　　者：刘志刚[1] 刘玉姣[1,2] 卢迈新[1] 朱华平[1] 可小丽[1] 高风英[1] 曹建萌[1] 王淼[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所农业部热带亚热带水产资源利用与养殖重点实验室,广东广州510380 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《上海海洋大学学报》2015年第2期182-189,共8页Journal of Shanghai Ocean University

基　　金：十二五国家科技支撑计划项目(2012BAD26B03-02);现代农业产业技术体系专项资金(CARS-49);广东省海洋渔业科技推广专项(A201101C02)

摘　　要：为了从分子水平评估莫桑比克罗非鱼、荷那龙罗非鱼及其正交子代（莫桑比克罗非鱼♀×荷那龙罗非鱼）和反交♂子代（荷那龙罗非鱼♀×莫桑比克罗非鱼）的耐盐性能,利♂用RT-PCR方法研究了盐胁迫下这4种罗非鱼不同组织中（鳃、肾、垂体和肌肉）热休克蛋白70（HSP70）基因的表达规律。结果表明：在盐度为22条件胁迫下4种罗非鱼各组织中HSP70表达水平总体呈现先增加后降低的规律,24 h时达最大值,48 h时下降至初始水平;盐度提高至35后HSP70的表达无显著变化。但不同种罗非鱼及不同组织之间也存在差异,反交子代鳃中HSP70在整个盐胁迫过程中都未上调表达;在盐度为22条件胁迫下,正反交子代肾中HSP70基因表达量比父母本肾中HSP70更早达到峰值;反交子代垂体中HSP70表达量在0~48 h内持续缓慢增加,48 h时达最大值;莫桑比克罗非鱼肌肉中HSP70基因在盐度22条件胁迫下表达量迅速升高,且维持较高水平直至盐度35条件胁迫之后。结果表明：HSP70表达水平的高低与罗非鱼的耐盐能力和应激程度密切相关,其中盐胁迫下肾中HSP70的表达规律与以上4种罗非鱼的耐盐能力之间相关性较强,可以作为评价这4种罗非鱼耐盐能力的潜在分子指标。To evaluate the salt tolerance of four strains of tilapia Oreochromis mossambicus, 0. hornorum, cross hybrid （ 0. mossambicus ♀× 0. hornorum ♂ ） and reciprocal cross hybrid （ 0. hornorum ♀×0. mossambicus ♂ ） at molecular level, expression profile of heat shock protein 70 gene （HSP70） in four tissues （gill, kidney, pituitary and muscle） of the four strains of tilapia under salt stress was studied using RT-PCR method. The results showed that at salinity 22, HSP70 gene expression in the four tissues of those tilapias increased first, peaked at 24 h and then dropped to initial level at 48 h, and there was no significant change in its expression level when the salinity was increased to 35. There were differences in the expression level of HSP70 gene among different strains of tilapia and among the four tissues under the treatment of salt stress. The expression level of HSP70 gene was not up-regulated in the gill of cross hybrid throughout the treatment. The expression level of HSP70 gene peaked earlier in the kidneys of cross and reciprocal cross hybrids compared to that in the parents at salinity 22. The expression level of HSP70 gene increased slowly from Oh to 48h, and peaked at 48h in the pituitary of reciprocal cross hybrid at salinity 22. The expression level of HSP70 gene in the muscle of O. mossambicus increased rapidly at salinity 22 and maintained high at salinity 35. In conclusion, the expression level of HSP70 gene was closely related to salt tolerance and stress level of tilapia. The expression level of HSP7O gene in the kidney of tilapia under salt stress was in positive correlation with their salt tolerance and it can be used as a potential molecular indicator for evaluating salt tolerance of tilapia.

关 键 词：莫桑比克罗非鱼(Oreochromis mossambicus) 荷那龙罗非鱼(O.hornorum) 正交子代 反交子代 盐胁迫 HSP70基因 

分 类 号：S917[农业科学—水产科学]