微囊藻毒素MC-LR对凡纳滨对虾细胞免疫相关基因表达水平的影响  

The toxicity impact of microcystin on expression of cellular immune-related genes in Litopenaeus vannamei

作　　者：傅一鸣[1] 李智[1] 柳峰松[2] 刘利平[1] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306 [2]河北大学生命科学学院,河北保定071002

出　　处：《上海海洋大学学报》2015年第2期196-202,共7页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(31101914);美国国际发展署Aqua Fish CRSP项目(3001325371)

摘　　要：对凡纳滨对虾进行血窦注射微囊藻毒素MC-LR染毒,取染毒前后肝胰腺及血细胞,采用Illumina Hiseq 2500测序平台进行基因表达谱分析,并对差异基因的基因本体(gene ontology,GO)注释和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路进行显著性富集分析。结果发现,酚氧化酶原、胰蛋白酶及C型凝集素等基因有显著的差异性表达。在GO富集性分析中发现细胞粘附显著性差异表达,细胞杀伤及细胞粘附分子结合物差异表达。KEGG富集性分析发现血细胞中吞噬体显著性差异表达,细胞凋亡、内吞作用和细胞粘附分子等通路呈表达差异。结果表明,凡纳滨对虾在被MC-LR染毒后,细胞免疫是抵御毒素的重要部分,其中细胞粘附和吞噬作用在抵御MC-LR对虾体的毒害过程中发挥了重要作用。In this article, Litopenaeus vannamei were challenged by microcystin-LR through injection. Shrimp hepatopancreas and blood samples were taken to analyze the transcriptome data using Illumina Hiseq 2500. The expression profiles were analyzed by differential expression analysis and enrichment analysis. The results showed that prophenoloxidase, trypsin, C-type lectin and other genes were significantly differentially expressed. In GO enrichment analysis, we found that cell adhesion, cell killing and cell adhesion molecule binding were differentially expressed, tn blood KEEG enrichment analysis, we found phagosome, apoptosis, endocytosis and cell adhesion molecules （CAMs） and other passways were significantly differentially expressed. Those results suggested that when L. vannamei was challenged by MC-LR, they would take a positive immune response to resist the infection, and cellular immune response was one of the crucial part. Phagocytosis and cell adhesion play important roles against the toxicity of MC-LR in the immune response.

关 键 词：凡纳滨对虾 微囊藻毒素 转录组 细胞免疫 

分 类 号：S917[农业科学—水产科学]