大鼠体内川芎多成分序贯代谢研究  

Study on Multicomponent Sequential Metabolism in Rats with Chuanxiong Rhizoma

作　　者：吕贝然[1] 杨文宁[1] 唐明敏[1] 隗丽[1] 马晓云[1] 罗志强[1] 刘洋[1] 

机构地区：[1]北京中医药大学,北京100102

出　　处：《中国中医药信息杂志》2015年第4期77-82,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81274042)

摘　　要：目的研究川芎单味药中多成分体内动态变化过程,阐明其体内代谢轮廓。方法采用高效液相色谱法建立川芎水提液指纹图谱,以此同时检测多成分变化情况。体内代谢采用封闭肠环法研究川芎口服后多成分经胃-肠-肝代谢变化过程。结果川芎水提液指纹图谱中监测到17种成分,在胃肠道稳定性研究中均基本稳定,其中有4种成分被肠道菌代谢,3种成分被肝代谢,2种新成分是肠代谢产物,1种新成分是肝代谢产物。结论采用序贯代谢,运用封闭肠环法研究中药口服后各成分代谢轮廓是可行的,为中药的代谢研究提供了实验依据。Objective To study the multicomponent in vivo dynamic process in Chuanxiong Rhizoma;To elaborate in vivo metabolic profiling. Methods HPLC was used to establish the fingerprint of aqueous extract of Chuanxiong Rhizoma, and multicomponent changes were detected at the same time. Closed-loop intestine method was used to study the multicomponent changes of oral administration of Chuanxiong Rhizoma after stomach-intestine-liver process. Results Totally 17 components were detected in the fingerprint of aqueous extract of Chuanxiong Rhizoma and they were basically stable in the digestive juice. For in vivo metabolism, 4 components were metabolized by intestinal flora;3 components were metabolized by liver;2 new components were the metabolites of intestinal flora;1 component was the metabolite of liver. Conclusion Multicomponent sequential metabolism and closed-loop intestine method were used to clarify that multicomponent metabolic profiling was feasible, and it could provide experimental basis for the metabolism of traditional Chinese medicine.

关 键 词：川芎 多成分 序贯代谢 封闭肠环 大鼠 

分 类 号：R284.1[医药卫生—中药学]