德国小蠊致敏原Bla g 2的Glu 233突变的分子对接研究  

Molecular Docking Studies on Point Mutation of Glu 233 from the German Cockroach Allergen Bla g 2

作　　者：杨铁[1] 王浩[2] 周波[1] 张春林[1] 

机构地区：[1]贵阳医学院生物学教研室,贵阳550004 [2]贵阳医学院生物技术教研室,贵阳550004

出　　处：《河南科学》2015年第3期359-363,共5页Henan Science

基　　金：贵州省教育厅自然科学研究项目(黔教合KY字(2012)039);2013年贵州省高等学校大学生创新创业训练计划(No.26)

摘　　要：为探讨Bla g 2的E233A突变对抗原抗体相互作用的影响,从RCSB数据库下载德国小蠊致敏原Bla g 2野生型及其单克隆抗体4C3的NMR结构,运用Swiss PDB Viewer将Bla g 2野生型(E233-93Q)构建为突变型(E233A-93Q)三维结构;并将Bla g 2野生型和突变型分别与其单克隆抗体4C3进行分子动力学模拟和蛋白-蛋白对接.结果表明:突变体E233A与单克隆抗体4C3结合能力下降,进而增加了与Ig E的结合力,可能加重过敏反应的发生.The present study was attempted to study the effect of immunoreaction which the mutagensis E233A from the German cockroach allergen Bla g 2. German cockroach wild-type Bla g 2 （E233-93Q） and monoclonal antibody 4C3 NMR structures were retrieved from RCSB database. The mutants （E233A-93Q） were obtained from Swiss PDB Viewer, and the monoclonal antibody 4C3 were used of molecular dynamics and protein-protein docking. The declined of binding capacity with allergen protein and monoclonal antibody after site-directed mutated, which would lead to increase with the IgE binding force and allergic reactions.

关 键 词：蟑螂 过敏 定向突变 分子动力学 蛋白-蛋白对接 

分 类 号：R392.8[医药卫生—免疫学]