HPLC测定亚贡叶中亚贡二萜酸A的含量  

Determination of smallanthaditerpenic acid A in leaves of Smallanthus sonchifolius by HPLC

作　　者：李德伟[1,2] 王晓彤[2] 窦德强[2] 董锋[3] 

机构地区：[1]天津天士力现代中药资源有限公司,北辰300402 [2]辽宁中医药大学药学院,大连116600 [3]珍奥集团股份有限公司,大连116600

出　　处：《药物分析杂志》2015年第3期406-408,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：辽宁省高等学校创新团队课题(LT2013020);辽宁省教育厅一般项目(L2013364)

摘　　要：目的：建立高效液相色谱法亚贡叶中亚贡二萜酸A的含量。方法：采用Agilent ZORBAX SB-C18（4.6 mm×150 mm,5μm）色谱柱,以乙腈-0.5%磷酸水溶液（35∶65）为流动相,流速1.0 m L·min-1,检测波长203 nm,柱温30℃。结果：亚贡二萜酸A的线性范围为0.064~1.028μg,r=0.999 9;加样回收率为99.6%,RSD为1.5%;批号为20101001、20101015、20101020的3批样品中亚贡二萜酸A含量分别为0.531、0.654、0.294 mg·g-1。结论：不同来源的亚贡叶的质量差异较大;方法学验证结果表明本法适用于亚贡叶的质量控制。Objective： To establish an HPLC method for determination of smallanthaditerpenic acid A in leaves of Smallanthus sonchifolius. Methods： The determination was performed by HPLC with Agilent ZORBAX SB- C18（ 4. 6mm × 150 mm,5 μm） column using acetonitrile- 0. 5% phosphate solution（ 35∶ 65） as the mobile phase,the flow rate was 1. 0 m L·min- 1,the detection wavelength was 203 nm and the column temperature was 30 ℃. Results：There was a good linear relationship with a range of 0. 064- 1. 028 μg for smallanthaditerpenic acid A,r = 0. 999 9;the average recovery rate of smallanthaditerpenic acid A was 99. 6%,RSD was 1. 5%. The contents of Smallanthus sonchifolius samples were 0. 531 mg·g- 1for lot No. 20101001,0. 654 mg·g- 1for lot No. 20101015,and 0. 294mg·g- 1for lot No. 20101020. Conclusion： The quality differences of Smallanthus sonchifoliu leaves from different sources are great; the results of method validation show that the method can be used to control quality of Smallanthus sonchifolius.

关 键 词：亚贡叶 亚贡二萜酸A 南美药食两用植物 雪莲果 雅龙果 YACON 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]