高效液相色谱法测定藏药砂生槐子中4种生物碱含量  

HPLC determination of fouralkaloids in Tibetan medicine Sophora moorcroftiana seeds

作　　者：胡春晖[1] 张发斌[1] 丁李[1] 刘阳[1] 

机构地区：[1]青海大学医学院,西宁810001

出　　处：《药物分析杂志》2015年第3期430-434,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：青海大学医学院中青年团队项目(2013-KT-1);青海省科技厅项目(2014-ZJ-716)

摘　　要：目的：运用高效液相色谱法分析砂生槐子中氧化苦参碱、槐果碱、槐定碱和苦参碱的含量。方法：改良砂生槐子中生物碱提取工艺,采用Diamonsil C18（2）（4.6 mm×250 mm,3μm）色谱柱,以0.02 mol·L-1磷酸缓冲盐（p H 8）-甲醇（55∶45）,流速1.0 m L·min-1,检测波长220 nm,柱温35℃。结果：氧化苦参碱、槐果碱、槐定碱和苦参碱质量浓度在0.46-59.00、0.40-51.60、0.55-70.00、0.59-75.00μg·m L-1的范围内线性良好,相关系数分别为0.999 9、0.999 9、0.999 9、0.999 8;加样回收率分别为99.98%、100.3%、99.69%、100.2%。氧化苦参碱、槐果碱、槐定碱和苦参碱4种生物碱的含量分别为59.37、0.99、0.26、15.35μg·g-1。结论：该方法经方法学验证可用于砂生槐子中生物碱的含量测定。Objective： To develop a method for the determination of matrine,oxymatrine,sophocarpine and sophoridine in Sophora moorcroftiana seeds by HPLC. Methods： Modified extract technology of total alkaloids in Sophora moorcroftiana seeds was adopted. The analytes were separated on a Diamonsil C18（ 2）（ 4. 6 mm × 250 mm,3 μm）column,eluted by 0. 02 mol·L- 1phosphate buffered saline（ p H 8） and 45% methanol（ 55∶ 45）,identified by UV detector at 220 nm at the column temperature of 35 ℃. Results： The linear ranges for oxymatrine,sophocarpine,sophoridine and matrine were 0. 46- 59. 00 μg·m L- 1（ r = 0. 999 9）,0. 40- 51. 60 μg·m L- 1（ r = 0. 999 9）,0. 55- 70. 00 μg·m L- 1（ r = 0. 999 9）,0. 59- 75. 00 μg·m L- 1（ r = 0. 999 8） μg·m L- 1,the average recoveries（ n = 6） were 99. 98 %,100. 3 %,99. 69 %,100. 2 %,while the sample content was 59. 37 μg·g- 1,0. 99 μg·g- 1,0. 26 μg·g- 1,15. 35 μg·g- 1,respectively. Conclusion： The experiment results indicate that the method can be used for the determination of matrine,oxymatrine,sophocarpine and sophoridine in Sophora moorcroftiana seeds.

关 键 词：西藏狼牙刺 砂生槐子 苦参碱 氧化苦参碱 槐果碱 槐定碱 高效液相色谱 藏药质量检测 

分 类 号：R917[医药卫生—药物分析学]