黄花美冠兰花芽分化过程中假鳞茎的代谢特征研究  

Study on Metabolic Characteristics of Pseudobulbs of Eulophia flava During the Floral Bud Differentiation

作　　者：王存[1] 尹俊梅[1] 杨光穗[1] 黄素荣[1] 张欢[1,2] 张志群[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所 农业部华南作物基因资源与种质创制重点开放实验室,海南儋州571737 [2]海南大学园艺园林学院,海南海口570228

出　　处：《热带作物学报》2015年第3期546-550,共5页Chinese Journal of Tropical Crops

基　　金：2012年国家公益性农业行业科技项目(No.201203071);农业生物资源保护与利用项目(No.2130135);2011公益性行业科研专项经费“国家重点保护野生花卉人工驯化繁殖及栽培技术研究与示范”(No.201203071)

摘　　要：采用生化测定的方法对黄花美冠兰[Eulophia flava(Lindley)Hooker f.]花芽分化过程中假鳞茎内可溶性糖、可溶性蛋白质和总核酸含量的变化进行研究。结果表明:整个花芽分化过程中可溶性糖和可溶性蛋白含量均呈先下降后大幅上升的变化趋势,C/N呈先大幅降低后缓慢升高,总核酸含量的变化呈先降低后升高再降低,且波动幅度较大。表明假鳞茎中可溶性糖、可溶性蛋白质和总核酸的代谢与黄花美冠兰花芽分化紧密相关。Abstra ct Changes of soluble sugar, soluble protein and nucleic acid contents in pseudobulbs of E. flava were studied by biochemical measurements during the flower bud differentiation. The results demonstrated： during the flower bud differentiation, soluble sugar and soluble protein contents in pseudobulbs reduced slightly at first and then increased rapidly. Changes of C/N value of pseudobulbs showed a sharp decline initially and followed by a slow rise. Changes of nucleic acid contents in pseudobulbs showed high-low-high-low and the fluctuation was obvious. The results indicated that metabolism of soluble sugar, soluble protein and nucleic acid closely related to flower differentiation.

关 键 词：黄花美冠兰 花芽分化 糖 蛋白质 核酸 

分 类 号：S682.31[农业科学—观赏园艺]