不同疏果处理下香草兰果荚脱落及其内源激素含量变化研究  

Pod Abscission and Changes of Endogenous Hormones in Vanilla Pod Under Different Fruit Load

作　　者：顾文亮[1,2,3] 陈娅萍[1] 王辉[1,2,3] 庄辉发 王华[1,2,3] 朱自慧[1,2,3] 宋应辉[1,2,3] 

机构地区：[1]中国热带农业科学院香料饮料研究所,海南万宁571533 [2]农业部香辛饮料作物遗传资源利用重点实验室,海南万宁571533 [3]海南省热带香辛饮料作物遗传改良与品质调控重点实验室,海南万宁571533

出　　处：《热带作物学报》2015年第3期551-556,共6页Chinese Journal of Tropical Crops

基　　金：科技支撑计划项目"香草兰规范化种植基地及其SOP优化升级研究"(No.2011BAI01B07);海南省中药现代化专项"香草兰良种繁育及规范化规模化栽培技术研究"(No.ZY201321)

摘　　要：香草兰果荚发育中存在严重的果荚脱落现象,对香草兰果荚脱落情况及其內源激素含量变化进行探讨,以期解决目前生产上果荚脱落率高的问题。设疏果后保留1个、8~10个香草兰果荚以及未疏果共3种处理,从果荚发育至脱落的60 d内分期取材,采用酶联免疫法分别测定各处理果荚中生长素（IAA）、赤霉素（GA）、玉米素核苷（ZR）和脱落酸（ABA）的含量。结果表明：3种处理的果荚脱落率与保留果荚数目呈正相关;IAA和ZR的含量在前期较高,ABA的含量在中、后期较高,GA的含量一直维持在较低的水平;较高的IAA/ABA和（IAA＋GA＋ZR）/ABA比值有利于控制果荚脱落;疏果处理有利于坐果的激素平衡,而保留过多的果荚则不利于形成激素平衡。本研究可为解决生产中的香草兰落荚问题提供理论依据和技术方法。Po d abscission in Vanilla is very serious during pod development. In order to solve this problem,the changes of endogenous plant hormones and pod abscissions under different fruit load were examined in this study. Three levels of fruit load after pollination were set by fruit thinning i.e. 1, 8-10 and all vanilla pods were retained as control. The contents of IAA, GA, ZR and ABA in pods from day 0 to day 60 after pollination were measured using enzyme-linked immunoassay. The results showed that the pod abscission rates of three fruit load treatments were positively correlated with the numbers of pods that retained. The contents of IAA and ZR were higher in earlier stage. while ABA content was higher in the middle and late stage, and GA content maintained a relatively low level.The higher ratio of IAA/ABA and（IAA ＋GA ＋ZR）/ABA were related to less pod abscission.Proper adjustment of fruit load may generated a hormone balance for pod retention. This study will provide theoretical basis for solving the problem of vanilla pod abscission during its production.

关 键 词：香草兰 果荚脱落 内源植物激素 

分 类 号：S682.31[农业科学—观赏园艺] S311[农业科学—园艺学]