喜树碱诱导小鼠皮层神经元凋亡的实验研究  

An Experimental Study on the Neuronal Apoptosis of Campotothecine Induces in mice1

作　　者：万亮琴[1] 刘亚明[2] 李芳赫 李峰[1] 臧妍妍[1] 张赛[1] 姜昭研 马家宝[1] 胡艳红[1] 李卫红[1] 罗广彬[1,3] 

机构地区：[1]北京中医药大学,北京100029 [2]北京航空航天大学,北京100191 [3]凯斯西储大学,美国俄亥俄州44106

出　　处：《中医药导报》2015年第7期26-30,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(81273885);北京中医药大学"航海中医药"协同创新项目(522/0100604299)

摘　　要：目的:观察喜树碱对体外原代培养小鼠神经元的神经毒性作用。方法:利用原代培养的小鼠神经元,首先观察喜树碱对神经元活性影响的量效、时效关系。然后,将半数致死量的喜树碱作用于神经元后,通过TUNEL法、γ-H2AX免疫荧光标记、流式细胞法,检测神经元发生凋亡的情况。结果:喜树碱可明显抑制神经元的细胞活性,且呈浓度和时间依赖性。与对照组比较,喜树碱组神经元γ-H2AX和TUNEL阳性染色细胞明显增多;流式细胞仪检测,喜树碱组早期凋亡(27.8%)和晚期凋亡(4.7%)的发生率也显著增高。结论:喜树碱能抑制神经元活性,引起神经元DNA损伤,诱导神经元发生凋亡,其神经毒性的内在机制有待进一步研究。Objective：To observe camptothecin neurotoxic effect of primary cultured mouse neurons in vitro. Methods：Firstly, observe the relationship between dose-effect and time-effect of Camptothecin effect on neuron activity. Then, by TUNEL stain- ing method, gamma H2AX immunofluorescence labeling and flow cytometry method, to detect of the neuron apoptosis inter- vened by Camptothecin. Results： Camptothecin inhibits obviously neuron cell activity and dependences on concentration and time. Compared with the control group, the camptothecin group neuron shows more gamma H2AX and TUNEL positive staining. Flow cytometry shows the increasing of early apoptosis （27.8%） and the late apoptosis （4.7%） in the camptothecin group. Conclusion：Camptothecin inhibits activity of neuron, causes DNA damage, and induced neuronal apoptosis. However, the internal mechanism of camptothecin neurotoxicity needs further research.

关 键 词：喜树碱 神经元 凋亡 神经毒性 

分 类 号：R285.5[医药卫生—中药学]