白术内酯对IEC-6细胞及食管癌细胞ECA9706增殖能力的影响  

Research of atractylenolide on proliferation ability of IEC-6 cells and esophageal cancer ECA9706 cells

作　　者：高小玲[1] 汪保英[1] 尹素改[1] 张钟允[1] 陈玉龙[1] 

机构地区：[1]河南中医学院,郑州450008

出　　处：《中华中医药杂志》2015年第3期921-923,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：河南中医学院博士启动基金(No.2010BSJJ-12);国家自然科学基金(No.81173177);河南省教育厅科学自然基金(No.2010B360004)~~

摘　　要：目的:观察白术内酯Ⅰ、Ⅱ、Ⅲ对IEC-6细胞及食管癌细胞ECA9706增殖能力的影响。方法:细胞种植于96孔板,24h后分别加入白术内酯Ⅰ、Ⅱ、Ⅲ200、100、50、25、12.5、6.25μg/m L,放置培养箱48h后,MTT法检测白术内酯Ⅰ、Ⅱ、Ⅲ不同剂量对IEC-6细胞及ECA9706细胞增殖能力的影响。结果:白术内酯Ⅰ和白术内酯Ⅲ随着剂量的增加,呈现从增殖到抑制的作用,其中以白术内酯Ⅰ50μg/m L对IEC-6细胞增殖率效果最佳,白术内酯Ⅱ小剂量对IEC-6细胞作用不明显,但是随着剂量的增加,呈现抑制作用;白术内酯Ⅰ、Ⅱ、Ⅲ200、100、50μg/m L均能抑制ECA9706细胞的增殖,其中以白术内酯Ⅱ效果明显。结论:白术内酯Ⅰ可能是白术健脾的有效成分,而白术内酯Ⅱ可能是抑制肿瘤细胞增殖的主要成分之一。Objective： To observe the proliferation effect of atractylenolide Ⅰ, Ⅱ, Ⅲ on IEC-6 cells and ECA9706 cells. Methods： IEC-6 cells and ECA9706 cells were seeded in 96-well plates, and after 24 hours, respectively added atractylenolide Ⅰ, Ⅱ, Ⅲ at concentrations of 200, 100, 50, 25, 12.5, 6.251ag/mL, then continued to incubate for 48h, the proliferation ability of IEC-6 cells and ECA9706 cells were detected by MTT assay. Results： Atractylenolide I and HI showed different action from proliferation to suppress with increasing doses, atractylenolide at 50μg/mL had the best proliferation effect on IEC-6 cells, small dose of atractylenolide Ⅱ had no obvious effect on IEC-6 cells, it showed inhibition effect when the dose increased. Atractylenolide Ⅰ, Ⅱ, Ⅲ at 200, 100, 50μg/mL could inhibit the proliferation of ECA9706 cells. And the effect of atractylenolide Ⅱ was obvious. Conclusion： Atractylenolide I may be the main active ingredient in strengthening the spleen, atractylenolide Ⅱ may be one of the main ingredients in inhibiting tumor cells proliferation.

关 键 词：白术内酯 增殖 IEC-6细胞 ECA9706细胞 

分 类 号：R285[医药卫生—中药学]