大鼠骨髓间充质干细胞及不同组织中17β-雌二醇的表达情况  

Study on the concentration of 17β-estradiol in BMSCs and different tissues of rats

作　　者：王俊玲[1] 黄思敏[1] 魏秋实[1] 谭新[1] 邓伟民[1] 

机构地区：[1]广州军区广州总医院华侨楼高级医疗中心,510010

出　　处：《实用医学杂志》2015年第6期905-907,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:81273778;81302994);广东省自然科学基金(编号:S2013040014927)

摘　　要：目的:通过检测17β-雌二醇在大鼠骨髓间充质干细胞(BMSCs)和不同组织中的表达情况,初步探索非性腺源性雌激素的分泌情况,为进一步研究BMSCs的生理功能以及绝经后骨质疏松的发病机制提供依据。方法:Elisa法检测0、24、48 h时BMSCS培养上清及细胞裂解物中的17β-雌二醇水平。选取SD大鼠不同组织,称重后物理破碎,Elisa法检测不同组织中的17β-雌二醇含量。结果:48 h培养上清中17β-雌二醇浓度较24 h培养上清中明显升高(P<0.05)。卵巢及软骨组织中17β-雌二醇的浓度高于其他组织,但不具有明显统计学差异(P>0.05)。结论:大鼠BMSCs可自身合成及分泌17β-雌二醇,其浓度在一定范围内与时间成正比,但对其自身或周围组织是否具有自分泌或旁分泌作用,还有待进一步研究。大鼠不同组织中17β-雌二醇浓度无明显差异,说明其旁分泌现象可能普遍存在且无性腺依赖性,但是否由该被测位点分泌尚需要进一步研究。这种非性腺源性的雌激素可能会成为绝经后参与骨代谢并维持女性骨骼健康的主要雌激素来源。Objective To investigate the concentration of 17β- estradiol in bone marrow stem cells（BM SCs） and different tissues of rats, and study the function of estradiol produced in extragonadal sites preliminarily. Methods（1） The concentration of 17β-estradiol in lysate of BMSCs and in supernatant cell culture media were detected by Elisa method after rats BMSCs were serum-free cultured in 0 h, 24 h and 48 h respective ly.（2） The tissues of organs were grinded and broken under ultrasonic wave, then washed and weighed. The concentration of 17β-estradiol in different tissues was detected by Elisa method. Results Compared with the concentration of 17β-estradiol at 24 h, the concentration of 17β-estradiol at 48 h significantly increased（P 〈0.05）. The concentration of 17β-estradiol among different tissues were higher, but the difference is not significant（P 〉0.05）.Conclusions（1） 17β-estradiol can be secreted by BMSCs, and the concentration is proportional to the time to some degree. But whether the extragonadal estrogen can function locally is still unclear.（2） The concentration of17β-estradiol is non- gonad dependent but whether it is secreted locally remains to be elucidated. The non- gonad estrogen could be the estrogen source of bone metabolism in order to sustain bone health after menopause.

关 键 词：骨质疏松症 17Β-雌二醇 骨髓间充质干细胞 

分 类 号：R580[医药卫生—内分泌]