化浊解毒方对反流性食管炎大鼠一氧化氮、血管活性肠肽及P物质的影响  

Influence of Huazhuo Jiedu Formula on Nitric Oxide,Vasoactive Intestinal Peptide and Substance P of Rats with Reflux Esophagitis

作　　者：赵艳[1] 霍永利[1] 李博林[2] 

机构地区：[1]河北省中医院 [2]河北医科大学

出　　处：《中医杂志》2015年第7期607-610,共4页Journal of Traditional Chinese Medicine

基　　金：河北省中医药管理局科研计划项目(2013046)

摘　　要：目的探讨化浊解毒方治疗反流性食管炎的作用机制。方法将60只Wistar大鼠随机分为空白组、模型组、西药组、中药组,每组15只。除空白组外,其余3组采用"不全幽门结扎+贲门肌切开术"配合湿热环境加高脂高糖饮食的综合方法制备反流性食管炎大鼠模型。西药组给予奥美拉唑0.36 mg/100 g灌胃,中药组予化浊解毒方1.25 g/100 g灌胃,空白组、模型组予蒸馏水1 ml/100灌胃。治疗8周后观察大鼠食管黏膜的组织病理变化,检测食管组织一氧化氮(NO)浓度及食管黏膜血管活性肠肽(VIP)、P物质(SP)水平。结果中药组与西药组均能改善模型大鼠食管黏膜组织病理变化,与模型组比较差异有统计学意义(P<0.05)。中药组NO水平降低,SP表达升高,与模型组、西药组比较,差异有统计学意义(P<0.05)。中药组VIP表达降低,与模型组比较差异有统计学意义(P<0.05),与西药组比较,差异无统计学意义(P>0.05)。结论化浊解毒方可能是通过升高SP表达,降低NO浓度及VIP表达,对反流性食管炎起到治疗作用。Objective To explore the mechanism of Huazhuo Jiedu Formula on reflux esophagitis. Methods Sixty normal Wistar rats were equally divided into blank control group,model group,western medicine treatment group and Chinese medicine treatment group. Three groups other than blank control group were all made reflux esophagitis models by a comprehensive method encompassing subtotal pylorus ligation,Heller myotomy,feeding damp-heat environment and high lipid and high sugar diet. After modeling,western medicine treatment group was intragastrically administrated by 0. 36 mg /100 g given omeprazole and traditional Chinese medicine treatment group was intragastrically administrated by 1. 25 g /100 g given Huazhuo Jiedu Formula,blank control group and model group were intragastrically administrated with distilled water. After 8 weeks,histopathological changes in esophagus tissues were observed.Meanwhile,the content of Nitric oxide（ NO） in esophageal tissue and the levels of vasoactive intestinal peptide（ VIP） and substance P（ SP） in esophagus tissues were tested. Results Pathological changes of esophageal mucosa were improved in both treatment groups,and the difference was statistically significant compared with model group（ P〈 0. 05）. Decrease of NO concentration and increase of SP in Chinese medicine treatment group were significant difference（ P〈 0. 05） compared with the model group and western medicine treatment group. Decrease of VIP in the Chinese medicine treatment group was significantly different（ P 〈0. 05） compared with the model group,but was not significantly different（ P 〉0. 05） compared with the western medicine group. Conclusion By means of elevating the levels of SP and reducing the levels of NO and VIP in esophagus tissues,Huazhuo Jiedu Formula can serve therapeutic effect on reflux esophagitis.

关 键 词：化浊解毒方 反流性食管炎 一氧化氮 血管活性肠肽 P物质 

分 类 号：R285.5[医药卫生—中药学]