紫茎泽兰乙醇提取物小鼠灌胃给药LD_(50)测定  

LD_(50) Determination of Ethanol Extracts of Eupatorium Adenophorum Spreng after Intragastric Administration in Mice

作　　者：杨红芹[1] 张旭强[1] 董寿堂[1] 龚晓敏[1] 

机构地区：[1]保山中医药高等专科学校,云南保山678000

出　　处：《中国药物警戒》2015年第3期133-136,共4页Chinese Journal of Pharmacovigilance

基　　金：云南省教育厅科学研究基金一般项目(2013Y534)

摘　　要：目的：测定紫茎泽兰乙醇提取物的半数致死量。方法取昆明种小鼠灌胃给药，首先通过预实验，找出紫茎泽兰乙醇提取物的0%和100%致死剂量（Dmin、Dmax），以此作为依据，计算正式实验分组的组距；再通过正式实验观察并详细记录动物的反应和死亡情况，选择统计学软件 SPSS 17.0机率单位加权回归法（Bliss）法计算 LD50。结果紫茎泽兰的 LD50为126.3 g·kg^-1，95%可信区间为107.8-146.6 g·kg^-1。结论紫茎泽兰毒性较大。Objective To measure the LD50 of ethanol extracts of Eupatorium adenophorum Spreng. Methods Kunming mice were given the ethanol extracts of Eupatorium adenophorum Spreng by intragastric administration. Through the pre experiment, to find out the 0% and 100% lethal dose （Dmin , Dmax） of ethanol extracts of Eupatorium adenophorum Spreng, as a basis for the formal test, to calculate the group distance of experimental group. The detailed reactions and death results of mice were observed and recorded through the formal test, LD50 was calculated with probit weighted regression method （Bliss） by the SPSS 17.0 statistics software. Results LD50 of Eupatorium adenophorum Spreng was 126.3 g·kg^-1, and 95% confidence interval was 107.8 -146.6 g·kg^-1. Conclusion The toxicity of Eupatorium adenophorum Spreng is higher.

关 键 词：紫茎泽兰 小鼠 灌胃 半数致死量 

分 类 号：R965[医药卫生—药理学] R289[医药卫生—药学]