化湿汤结合润肤止痒膏对湿疹小鼠细胞免疫功能的影响  

Effects of Huashi Decoction and Runfuzhiyang Cream on Cell Immunologic Function in Mice with Eczema

作　　者：李春光 王月飞[2] 赵红晔[2] 李辉[3] 刘彬 孙莹 董辉 刘萌 刘晶 王滨[2] 

机构地区：[1]齐齐哈尔市中医医院,黑龙江齐齐哈尔161000 [2]齐齐哈尔医学院,黑龙江齐齐哈尔161006 [3]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中国药物警戒》2015年第3期137-139,143,共4页Chinese Journal of Pharmacovigilance

基　　金：齐齐哈尔市科学技术局社会发展项目(SFZD-2012005)

摘　　要：目的观察湿疹小鼠动物模型外周血中的CD4+和CD8+的水平,分析化湿汤结合润肤止痒膏对湿疹小鼠CD4+、CD8+及其比值的影响,探讨化湿汤结合润肤止痒膏治疗湿疹的机制。方法将60只健康成年昆明种小鼠随机分为空白对照组、湿疹模型组、湿疹阳性药物组、化湿汤组、润肤止痒膏组及化湿汤结合润肤止痒膏组,除空白对照组外,其余各组建立湿疹模型。空白对照组和湿疹模型组不进行治疗;湿疹阳性药物组用醋酸曲安奈德益康唑乳膏治疗,而化湿汤组、润肤止痒膏组及化湿汤结合润肤止痒膏组则采用相应药物治疗,每日1次。通过流式细胞仪对小鼠外周血中T淋巴细胞亚群进行测定。结果与湿疹模型组相比,湿疹阳性药物组、化湿汤组、润肤止痒膏组及化湿汤结合润肤止痒膏组小鼠外周血中CD4+T淋巴细胞百分比及CD4+T/CD8+T均明显提高(P均<0.01),CD8+T淋巴细胞百分比显著下降(P均<0.01)。与湿疹阳性药物组相比,化湿汤组、润肤止痒膏组小鼠外周血CD4+T淋巴细胞百分比及CD4+T/CD8+T均明显降低(P均<0.01),CD8+T淋巴细胞百分比显著升高(P均<0.01);而化湿汤结合润肤止痒膏组小鼠外周血CD4+T淋巴细胞、CD8+T淋巴细胞百分比及CD4+T/CD8+T无差异(P>0.05)。与化湿汤组、润肤止痒膏组相比,化湿汤结合润肤止痒膏组对小鼠外周血中CD4+T淋巴细胞百分比、CD8+T淋巴细胞百分比及CD4+T/CD8+T的作用有显著差异(P<0.01或<0.05)。结论化湿汤结合润肤止痒膏可能是通过提高CD4+T百分比,降低CD8+T淋巴细胞百分比以及提高CD4+/CD8+,纠正了CD4+T淋巴细胞相对减少,CD8+T淋巴细胞百分比相对增多,恢复了湿疹小鼠的细胞免疫功能和纠正了免疫失调。Objective To observe the peripheral blood CD4^＋ and CD8^＋ of animal models in mice with eczema, analyze the influence of Huashi decoction combined with Runfuzhiyang cream for CD4^＋, CD8^＋ and its ratio in mice with eczema and explore the mechanism of Huashi decoction combined with Runfuzhiyang cream. Methods 60 healthy adult Kunming mice were randomly divided into blank control group, eczema model group, eczema positive drug group, Huashi decoction group, Runfuzhiyang cream group and Huashi decoction combined with Runfuzhiyang cream group, eczema models were established in all groups except for the blank control group. Blank control group and eczema model group were not given treatment. Eczema positive drug group was treated by triamcinolone acetonide acetate econazole cream. Huashi decoction group, Runfuzhiyang cream group and Huashi decoction combined with Runfuzhiyang cream group were treated by corresponding drug, 1 times daily. The T lymphocyte subpopulation in the mice peripheral blood was determined by flow cytometry. Results Compared with eczema model group, mice peripheral blood CD4^＋ T lymphocytes＇ percentage and CD4^＋/CD8^＋ T significantly increased （P all〈 0.01）, and CD8^＋ T lymphocytes＇ percentage decreased significantly （P all〈0.01） in eczema positive drug group,Huashi decoction group, Runfuzhiyang cream group and Huashi decoction combined with Runfuzhiyang cream group. Compared with eczema positive drug group, mice peripheral blood CD4^＋T lymphocytes＇ percentage and CD4^＋/CD8^＋ T were significantly lowered （P 〈0.01）, CD8^＋ T lymphocytes＇ percentage increased significantly （P 〈0.01）in Huashi decoction group and Runfuzhiyang cream group; while mice peripheral blood CD4^＋ T lymphocytes, CD8^＋ T lymphocytes＇ percentage and CD4^＋T/CD8^＋ T had no difference（P〉0.05） in Huashi decoction combined with Runfuzhiyang cream group. Compared with Huashi decoction group and Runfuzhiyang cream group, mice peripheral blood CD4^＋ T lymphocytes�

关 键 词：湿疹 化湿汤 润肤止痒膏 细胞免疫 

分 类 号：R965[医药卫生—药理学] R289[医药卫生—药学]