高海拔环境对大鼠海马S100B蛋白表达的影响及意义  

THE EFFECT AND SIGNATURE OF HYPOXIA ON THE EXPRESSION OF S100B PROTEIN AT HIPPOCAMPUS IN RATS

作　　者：张帅[1] 黄明玉[1] 祁存芳[1] 李福鑫[1] 张军峰[2] 张建水[3] 

机构地区：[1]青海大学医学院基础医学部解剖学教研室,青海西宁810001 [2]西安医学院人体解剖学教研室,陕西西安710021 [3]西安交通大学医学院,教育部环境和基因相关疾病重点实验室,神经生物研究所,陕西西安710061

出　　处：《青海医学院学报》2015年第1期15-19,共5页Journal of Qinghai Medical College

基　　金：青海省应用基础研究资助项目(No.2013-Z-727);青海大学医学院科研团队项目(2013-KT-002)

摘　　要：目的观察高海拔环境下大鼠脑组织海马区的细胞形态结构变化和S100B蛋白的表达,探索缺氧刺激后脑组织损伤和修复的机制。方法 120只健康成年SD大鼠,随机分成两组:模拟海拔5 000米组(实验组:H组)和海拔2 200米组(对照组:M组);H组经模拟海拔5 000米低压氧舱预处理3天,转移至海拔2 200米环境下饲养,M组不经模拟海拔5 000米低压氧舱预处理,直接在海拔2 200米环境饲养。两组又分为缺氧损伤后较低海拔环境恢复1、3、7、14、28天五个亚组,在相应时间点取脑组织,分别用Nissl、免疫组织化学染色方法染色,显微镜下观察海马区细胞结构,进行阳性细胞计数,并对相关结果进行分析。结果 Nissl染色可见M组大鼠海马区神经元排列较整齐、结构完整,未见明显损伤细胞;H组大鼠海马区神经元细胞数量减少、排列紊乱,其中H组1天和3天亚组出现明显的组织水肿,出现变性和核浓缩的损伤细胞;S100B免疫组化染色阳性细胞计数结果显示,H组S100B蛋白的表达在低压缺氧刺激后第1、3天明显高于M组对应时间点(P<0.05),其他时间点S100B蛋白表达量无统计学差异。结论高海拔低压缺氧环境下高表达的S100B蛋白诱导大鼠海马细胞形态和组织结构损伤。Objective To observe the hippocampus structure change and S100 B protein expression under high altitude environment,to explore the mechanisms of brain injury and restore after hypobaric hypoxia. Methods 120 SD rats were randomly divided into 2 groups： group H： high altitude group（ 5000m）; group M： control group（ 2200m）. Group H was pretreated 3 days in the low pressure oxygen tank to simulate the 5 000 meters environment,then shift them to 2 200 meters environment. Both group were divided into five subgroup after hypoxia injury：1d,3d,7d,14 d,and 28 d,respectively. Nissl staining and immunohistochemical staining to detect the hippocampus structure and positive cell count. Results Nissl staining in group M showed that the hippocampal neurons arranged neatly and structural integrity,no obvious damage cells; Group H was observed that the number of neurons decreased and disordered arrangement in hippocampus and subgroup 1d and 3d appeared obvious tissue edema; for the S100 B protein positive cell counting,after low pressure of hypoxia stimulation 1d or 3d,the positive cell number was significantly higher than that of group M with corresponding time points（ P〈 0. 05）. Conclusion S100 B protein is expressed higher level under hypobaric hypoxian and the increased S100 B protein may induce the morphological change and cell damage in hippocampus of rats.

关 键 词：低压缺氧 大鼠 海马 S100B 

分 类 号：R34[医药卫生—基础医学]