复方葶苈泻肺利水合剂中含生物碱类成分药材的提取工艺优选  

Optimization of Extraction Technology of Herbs Containing Alkaloids from Compound Tingli Xiefei Lishui Mixture

作　　者：王丽[1,2] 黄一平[2] 徐佳[1,2] 马亚萍[1,2] 刘小敏[1] 

机构地区：[1]南京中医药大学药学院,南京210046 [2]江苏省中医药研究院国家中医药管理局中药释药系统重点研究室,南京210028

出　　处：《中国实验方剂学杂志》2015年第7期27-30,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:考察不同提取方法中法半夏对黄连中盐酸小檗碱溶出率的影响。方法:选择复方葶苈泻肺利水合剂为考察对象,分别以水和乙醇为溶媒,采用方中法半夏、黄连、益母草、半边莲合提取及缺法半夏其余3味药材提取2种方法提取,比较提取液中盐酸小檗碱含量。以盐酸小檗碱提取量为指标,通过正交试验考察乙醇体积分数、提取时间、提取次数及料液比对复方葶苈泻肺利水合剂中4味药材提取工艺的影响。采用HPLC测定盐酸小檗碱含量,流动相乙腈-0.05 mol·L-1磷酸二氢钾溶液(50∶50)(每100 m L中加十二烷基硫酸钠0.4 g,以磷酸调节p H 4.0),检测波长345 nm。结果:以水为溶媒,2种方式提取的盐酸小檗碱含量差别较大,合提与缺法半夏提取时盐酸小檗碱平均质量分数分别为9.20,13.53 mg·g-1;以乙醇为溶媒,2种方式提取的盐酸小檗碱含量差别较小,平均质量分数分别为35.15,36.12 mg·g-1。4味药材的最佳提取工艺为加10倍量70%乙醇回流提取2次,每次1.5 h;盐酸小檗碱提取量37.92 mg·g-1。结论:以水为溶媒,法半夏与黄连合提将导致盐酸小檗碱含量显著降低;以乙醇为溶媒,法半夏与黄连合提对盐酸小檗碱溶出率影响较小,且在乙醇溶液中盐酸小檗碱提取率远高于水提法。优选的提取工艺稳定可行、重复性好,可用于复方葶苈泻肺利水合剂的工业化生产。Objective： To investigate effects of Pinelliae Rhizoma Praeparatum on dissolution rate of berberine in Coptidis Rhizoma by different extraction methods. Method： Taking compound Tingli Xiefei Lishui mixture for example, water and ethanol were selected as solvent, extraction methods included mixed extraction of four herbs and remaining three herbs without Pinelliae Rhizoma Praeparatum, then the content of berberine in extract were compared. With extracting amount of berberine as index, orthogonal test was used to optimize extraction technology with the concentration and amount of ethanol, extracting times and time as factors. HPLC was employed to determine the content of berberine with mobile phase of acetonitrile-0.05 mol · L^-1 potassium dihydrogen phosphate （50： 50） and detection wavelength at 345 nm. Result： In water extraction process, the content of berberine was significantly differernt, average contents of berberine from mixed extraction of four herbs and remaining three herbs without Pinelliae Rhizoma Praeparatum were 9.20, 13.53 mg g^-1, respectively. In ethanol extraction process, the content of bererine had little difference, which were 35.15, 36. 12 mg g^-1.Optimum ethanol extraction process was as follows： reflux extracted 2 times with 10 times the amount ethanol for 1.5 h each time ; extracting amount of berberine was 37.92 mg g^-1 Conclusion ： With solvent, when four herbs mixed extractiing, Pinelliae Rhizoma Praeparatum can reduce the content of significantly. With ethanol as solvent, when four herbs mixed extracting, Pinelliae Rhizoma Praeparatum of 70% water as bererine has little effect on the content of bererine, and ethanol extraction method has higher extracting rate than water extraction method. This optimized technology is stable and feasible with good repeatability, and it can be used in industrial production of compound Tingli Xiefei Lishui mixture.

关 键 词：复方葶苈泻肺利水合剂 提取方式 盐酸小檗碱 法半夏 黄连 

分 类 号：R283.6[医药卫生—中药学] R944.1[医药卫生—中医学]