化风丹中As_2S_2及As_2O_3的含量测定  

Determination of As_2S_2 and As_2O_3 in Huafeng Dan

作　　者：曹国琼[1,2] 张永萍[1] 徐剑[1] 高莉 廖小刚 

机构地区：[1]贵阳中医学院,贵阳550002 [2]北京师范大学,北京100875 [3]遵义廖元和堂药业有限公司,贵州遵义563000

出　　处：《中国实验方剂学杂志》2015年第7期54-56,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部中小企业创新基金项目(10C26215205058);国家火炬计划项目(2011GH021909);贵州省重大科技专项[黔科合重大专项字(2011)6019];贵州省火炬计划项目[黔科合高字(2010)5002]

摘　　要：目的：建立化风丹中不溶性砷盐和可溶性砷盐的含量测定方法,为该制剂的质量控制提供参考。方法：采用滴定法和紫外分光光度法分别测定18批化风丹中二硫化二砷和三氧化二砷的含量。结果：建立的二硫化二砷含量测定方法重复性好,平均加样回收率97.61%,RSD 2.0%。三氧化二砷在4-16μg与吸光度线性关系良好,平均回收率97.77%,RSD2.1%。18批化风丹中二硫化二砷质量分数0.012-0.021 3 g·g^-1,平均质量分数0.018 4 g·g^-1,差异系数13.3%;三氧化二砷质量分数75.24-124.55μg·g^-1,平均质量分数92.99μg·g^-1,差异系数15.3%。结论：建立的含量测定方法简单、准确,为化风丹质量标准提升提供实验依据。不同批次化风丹中二硫化二砷和三氧化二砷含量差异较大。Objective： To establish determinations of soluble arsenic salt and insoluble arsenic salt in Huafeng Dan in order to provide a reference for quality control of this preparation. Method： Contents of As2S2 and As2O3 in 18 batches of Huafeng Dan were determined by titration and UV spectrophotometry, respectively. Result ： This established determination of As2S2 was reproducible with average recovery of 97.61%. Linear range of As2O3 was 4-16 μg with average recovery of 98.42 %. In 18 batches of samples, mass fraction of As2S2 was 0. 012- 0. 021 3 g g^-1 with average value of 0. 018 4 gg^-1, difference coefficien was 13.3% ; mass fraction of As2O3 was 75.24-124.55μg·g^-1 with average value of 92.99μg·g^-1, difference coefficien was 15.3% . Conclusion： These determinations are simple and accurate, they can provide experimental basis for improvement of quality standards of Huafeng Dan. Contents of As2S2and As2O3 in different batches of samples are quite different.

关 键 词：化风丹 雄黄 二硫化二砷 三氧化二砷 

分 类 号：R283.6[医药卫生—中药学] R944.2[医药卫生—中医学]