HPLC测定跌打伤药凝胶膏剂中血竭素和延胡索乙素的含量  

Determination of Dracohodin and Tetrahydropulmatine in Dieda Shangyao Cataplasm by HPLC

作　　者：李江英 赵锋 吕卓 孙哲[3] 张亚军[3] 

机构地区：[1]西安市中医医院,西安710001 [2]陕西省食品药品检验所,西安710065 [3]西北大学生命科学学院,西安710069

出　　处：《中国实验方剂学杂志》2015年第7期57-60,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：陕西省科学研究发展计划项目(2013k12-07-06);西安市科技计划项目(sf1205)

摘　　要：目的：建立同时测定跌打伤药凝胶膏剂中血竭素和延胡索乙素的方法。方法：采用C18色谱柱（4.6 mm×250 mm,5μm）,流动相甲醇-0.1%磷酸水溶液（三乙胺调pH至6.0）（60∶40）,流速0.8 m L·min^-1,程序可变检测波长（0-20 min为440 nm,20-35 min为280 nm）,柱温30℃,进样量10μL。结果：血竭素和延胡索乙素分别在0.037 0-0.592 8,0.026 50-0.424 0μg峰面积与进样量呈良好的线性关系,平均回收率分别为98.1%（RSD 1.4%）,96.7%（RSD 1.7%）。结论：该方法简便、准确、重复性好,可用于跌打伤药凝胶膏剂的质量控制。Objective： To establish a method for simultaneous determination of dracohodin and tetrahydropulmatine in Dieda Shangyao cataplasm. Method： A high performance liquid chromatographic （HPLC） method was adopted with programmed wavelength UV detection. A C18 column （4.6 mm ×250 mm,5μm） was used for the analysis at 30 ℃. The separation was carried out with the mobile phase consisting of methanol and 0.1% phosphoric acid （60： 40, adjusted to pH 6.0 with triethylamine） at a flow rate of 0. 8 mL ·min^-1 , and the sample size was 10 μL. The eluate was detected by programmed wavelength （0-20 min, 440 nm; 20-35 min, 280 nm）. Result： The linearities were from 0. 037 0 to 0. 592 8 μg for dracohodin, 0. 026 5 to 0. 424 0 μg for tetrahydropulmatine. The average recoveries of dracohodin and tetrahydropulmatine were 98.1% （RSD 1.4% ） and 96.7% （RSD 1.7% ）, respectively. Conclusion： The established method was simple, accurate and highly reproducible that it could be used for quality control of Dieda Shangyao cataplasm.

关 键 词：跌打伤药凝胶膏剂 血竭素 延胡索乙素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]