HPLC同时测定清热定眩合剂中3种成分的含量  

Simultaneous Determination of Three Components in Qingre Dingxuan Mixture by HPLC

作　　者：许红玮[1] 刘轲[1] 张怀亮[1] 

机构地区：[1]河南中医学院第一附属医院,郑州450000

出　　处：《中国实验方剂学杂志》2015年第7期72-74,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省科技攻关计划项目(082102310080)

摘　　要：目的：建立HPLC同时测定清热定眩合剂中黄芩苷、新橙皮苷及丹酚酸B含量的方法。方法：采用Waters SunfireTMC18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-0.1%磷酸（21∶79）,流速l.0 mL·min^-1,柱温30℃,检测波长280nm。结果：黄芩苷、新橙皮苷及丹酚酸B的线性范围分别为0.08-0.79（r=0.999 9）,0.07-0.75（r=0.999 9）,0.04-0.19μg（r=0.999 8）;平均回收率分别为96.5%,96.9%,97.4%。结论：建立的HPLC方法简便,结果准确、可靠、重复性好,可用于清热定眩合剂的质量控制。Objective： To establish an HPLC method for simultaneous determination of baicalin; neohesperidin; salvianolic acid B in Qingre Dingxuan Mixture. Method： The chromatographic method was carried out on an Waters SunfireTM C18 （4.6 mm×250 mm,5μm） with acetonitrile-0. 1% phosphoricaeid solution as the mobile phase by gradient elution. The flow rate was 1 mL·min^-1. The detection was set at the wavelength of 280 nm. The column temperature was at 30 ℃. Result： The linear ranges of baicalin ; neohesperidin ; salvianolie acid B were0.08-0.79 μg （r=0.9999） , 0.07-0.75 μg （r =0.999 9） , 0.04-0. 19 μg （r =0. 999 8） respectively.The average recoeries were 96.5% , 96.9% , 97.4% respectively. Conclusion： The method is simple, accurate and reproducible.

关 键 词：高效液相色谱法 清热定眩合剂 黄芩苷 新橙皮苷 丹酚酸B 

分 类 号：R284.1[医药卫生—中药学]