丹参多酚酸对大鼠糖尿病脑缺血再灌注损伤恢复期相关基因表达的影响  

Effect of Salvianolic Acids on Related Gene Expression in Convalescent Period of Cerebral Ischemiareperfusion Injury in Diabetic Rats

作　　者：何前松[1,2] 马萌萌[1] 胡利民[1] 杨红云[1] 刘晓雷[1] 王乔悦 庄朋伟[1] 张艳军[1] 叶正良[3] 鞠爱春 

机构地区：[1]天津中医药大学中医药研究院,天津市现代中药重点实验室,省部共建国家重点实验室,天津市中药药理学重点实验室,天津300193 [2]贵阳中医学院,贵阳550002 [3]天津天士力之骄药业有限公司,天津300400

出　　处：《中国实验方剂学杂志》2015年第7期80-86,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家“重大新药创制”科技重大专项(2012ZX09101202);“十二五”期间天津市高等学校“创新团队培养计划”项目(TD12-5035)

摘　　要：目的：研究丹参多酚酸对大鼠糖尿病局灶性脑缺血再灌注损伤恢复期相关基因表达的影响,探索丹参多酚酸治疗糖尿病脑卒中的作用机制。方法：选用健康雄性Wistar大鼠,体重200-220 g,按60 mg·kg^-1剂量一次性腹腔注射链脲佐菌素（STZ）溶液建立糖尿病大鼠模型,造模成功后将大鼠随机分为假手术组、脑缺血再灌注模型组和丹参多酚酸治疗组,每组6只大鼠,采用线栓法建立局灶性脑缺血再灌注模型。丹参多酚酸治疗组于再灌注3 h后尾静脉注射（21 mg·kg^-1）,每日1次,连续给药14 d,假手术组和模型组给予同体积生理盐水。采用基因芯片技术检测脑组织中相关基因表达。结果：糖尿病大鼠脑卒中14 d后,模型组与假手术组比较差异表达的基因有67条,其中有41条基因上调,26条基因下调（P≤0.05,倍数变化（FC）≥2.0;丹参多酚酸治疗组与模型组比较差异表达的基因有64条,其中有45条基因上调,19条基因下调P≤0.05,FC≥2.0。结论：丹参多酚酸对糖尿病局灶性脑缺血再灌注损伤大鼠脑组织基因表达具有调控作用,为进一步研究注射用丹参多酚酸治疗糖尿病脑卒中的机制提供了新的线索。Objective： This paper studies the effect of salvianolic acids on the related gene expression in the convalescent period of cerebral ischemia-reperfusion injury in diabetic rats, in order to explore the mechanism. Method： The Wistar rats were randomly divided into control group and model group. The single intraperitoneal injection of streptozotocin （STZ） 60 mg ·kg^-1 was used to establish diabetes mellitus. After the modeling, the rats were randomly assigned to sham group （SH）, ischemia-reperfusion group （IR） and salvianolic acids treatment group, six rats in each group. The focal cerebral ischemia-reperfusion model was used. The rats in the salvianolic acids treatment group would receive tail vein injection （21 mg·kg^-1 ） after 3 hours of reperfusion, once per day for 14 days. The SH and the model group were injected with the same volume of saline. The related gene expression in the brain tissue was detected by the gene chip technique. Result： Fourteen days after the diabetic rats got a stroke, 67 differential expressed genes were detected, among the 41 genes were up-regulated and 26 genes were down-regulated [ P ≤0.05, fold chang （FC） ≥ 2.0 ] ; fourteen days after the treatment of salvianolic acids, compared with model group 64 differential expressed genes were detected, among the 45 genes were up-regulated and 19 genes were down-regulated （P≤0.05, FC ≥2.0）. Conclusion： Salvianolic acids exerts regulation on the rat＇s cerebral gene expression during the focal cerebral ischemia-reperfusion injury （CIRI） , which provides a new clue for the further study of the mechanism

关 键 词：糖尿病 局灶性脑缺血再灌注损伤 注射用丹参多酚酸 基因表达谱 

分 类 号：R285.5[医药卫生—中药学]