生品和蜜炙桑白皮对哮喘大鼠血清NO,LPO,IL-4和IFN-γ的影响  

Effect of Crude and Honeyed Mori Cortex on Serum NO,LPO,IL-4 and IFN-γ in Asthmatic Rats

作　　者：隋在云[1] 王爱洁[1] 李群[1] 

机构地区：[1]山东省中医药研究院,济南250014

出　　处：《中国实验方剂学杂志》2015年第7期95-98,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医药管理局"十二五"中医药行业科研专项(20110700719)

摘　　要：目的:探讨生品和蜜炙桑白皮对哮喘大鼠血清中一氧化氮(NO),脂质过氧化物(LPO),白细胞介素-4(IL-4)和干扰素-γ(IFN-γ)的影响。方法:Wistar大鼠随机分为空白组,模型组,阳性药地塞米松0.4 mg·kg-1组,生品和蜜炙桑白皮1.08,3.24 g·kg-1组。造模大鼠于实验第1,7天ip卵蛋白混悬液1 m L致敏,第15天起雾化吸入1%卵蛋白生理盐水,激发5d。从造模之日起开始ig给药,共20 d。观察大鼠10 min内出现Ⅱ级哮喘的潜伏期,肺泡灌洗液(BALF)中嗜酸粒细胞数(EOS),血清中NO,LPO,IL-4和IFN-γ的水平。结果:与空白组相比,模型组大鼠哮喘反应的潜伏期显著缩短,BALF中EOS及血清NO,LPO,IL-4含量增加(P<0.01),血清IFN-γ含量减少(P<0.001)。与模型组相比,桑白皮蜜炙品、生品3.24 g·kg-1剂量组大鼠显著延长哮喘反应的潜伏期、减少BALF中EOS及血清NO,LPO,IL-4含量(P<0.05)、升高血清IFN-γ(P<0.01)。桑白皮生品与蜜炙品之间各项指标均无显著差异。结论:桑白皮蜜炙品和生品均能明显降低哮喘大鼠BALF中炎性细胞数量,影响细胞因子水平变化,从而减轻气道炎症反应,改善哮喘症状。Objective： To investigate the effect of Mori Cortex before and after honey processing on serum nitric oxide （NO） , lipid peroxidation （LPO） , interleukin-4 （IL-4） and interferon （IFN-γ） of asthmatic rats, to study the anti-asthmatic mechanism of honeyed Mori Cortex. Method： Wistar rats were randomly divided into control group, model group, dexamethason 0.4 mg ·kg^-1 group, the crude and honey processed Cortex Mori 1.08, 3.24 g ·kg^-1 groups. Asthma rat model was established by sensitization and stimulation with ovalbumin （OVA） conjuncted with Al（OH） 3. The latent period of class Ⅱ asthma in 10 min, bronchial alveolar lavage fluid （BALF） eosinophil number, the levels of NO, LPO, IL-4 and IFN-γ in serum were determined. Result： Compared with the control group, the latent phase was significantly shorter, the BALF eosinophil number and the levels of NO, LPO, IL-4 in serum were significantly increased in the model group （P 〈 0.01 ）. The group could also significantly decreased the levels of IFN-γ （P 〈 0. 001 ）. Compared with the model group, the latent phase was significantly longer, the BALF eosinophil number and the levels of NO, LPO, IL-4 in serum were significantly reduced in honey processed Mori Cortex and crude Mori Cortex 3.24 g·kg^-1 groups （P 〈 0.05）. The groups could also significantly increased the levels of IFN-γ （P 〈 0.01 ）. Conclusion： The Mori Cortex before and after honey processing can significantly reduce the number of inflammatory ceils in BALF of asthmatic rat and impact the levels of cytokines, thereby improving airway inflammation in asthma and symptoms of bronchial asthma.

关 键 词：蜜炙 桑白皮 哮喘 一氧化氮 脂质过氧化物 白细胞介素-4 干扰素-γ 

分 类 号：R285.5[医药卫生—中药学]