林氏降脂方对高脂血症模型大鼠的降脂与肝保护作用  

Lipid-lowering and Liver Protection Effects of Lin's Jiangzhifang on Model Rats with Hyperlipoidemia

作　　者：张艳霞[1,2] 苏奋翔[3] 方耀武[3] 李明淑[3] 陈绍仪 安方玉[5] 

机构地区：[1]甘肃中医学院科研实验中心,兰州730000 [2]甘肃省中药药理与毒理学重点实验室,兰州730000 [3]甘肃省中医院白银分院,甘肃白银730900 [4]白银市第二人民医院,甘肃白银730900 [5]甘肃中医学院教学实验中心,兰州730000

出　　处：《中国药房》2015年第10期1336-1338,共3页China Pharmacy

基　　金：甘肃省中医药管理局科研项目(No.GZK-2013-70)

摘　　要：目的:研究林氏降脂方对高脂血症模型大鼠的降脂与肝保护作用。方法:取雄性SD大鼠随机分为正常对照(等容蒸馏水)组、模型(等容蒸馏水)组、阳性对照(脂脉康胶囊0.4 g/kg)组和林氏降脂方高、中、低剂量[12.962、6.481、3.241 g(生药)/kg]组,每组10只。正常对照组大鼠普通饲料喂养,其余各组大鼠高脂乳剂联合高脂饲料喂养以复制高脂血症模型;复制模型的同时ig给予相应药物,每日1次,连续6周。末次给药后,处死各组大鼠,检测其血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)水平,并观察其病理变化。结果:与正常对照组比较,模型组大鼠的TC、TG、LDL-C、ALT、AST水平均升高,HDL-C含量减少,差异具有统计学意义(P<0.01)。与模型组比较,阳性对照组和林氏降脂方高剂量组大鼠的TC、TG、LDL-C、ALT、AST水平均降低,HDL-C含量增加;林氏降脂方中、低剂量组大鼠的TC、TG含量减少,HDL-C含量增加;林氏降脂方中剂量组大鼠的AST活性减弱,以上差异均具有统计学意义(P<0.01)。病理切片显示,模型组大鼠肝细胞弥散浊肿、重度脂肪变性,林氏降脂方中、低剂量组大鼠轻度脂肪变性,林氏降脂方高剂量组和阳性对照组大鼠肝细胞基本正常。结论:林氏降脂方对高脂血症模型大鼠具有降脂作用,并能改善肝脏脂肪变性。OBJECTIVE：To study the lipid-lowering and liver protection effects of Lin＇s Jiangzhifang on model rats with hyperlipoidemia. METHODS：Male SD rats were randomly divided into normal control group（isovolumic distilled water）,model group（isovolumic distilled water）,positive control group（Zhimaikang capsules 0.4 g/kg）and the groups of high-dose,mediumdose and low-doses [12.962,6.481 and 3.241 g（medicinal materials）/kg] of Lin＇s Jiangzhifang,with 10 rats in each group. The rats in the normal control group were fed normal diets,while those in other groups were fed high-fat emulsions combined with high-fat diets to establish hyperlipidemic models;meanwhile,they were ig given corresponding drugs for 6 consecutive weeks,once a day. After the last administration,all rats were sacrificed to determine the total serum cholesterol（TC）,triglyeride（TG）,low-density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol（HDL-C）,aspartate transaminase（AST）and glutamic transaminase（ALT）levels;the pathological changes were observed. RESULTS：Compared with normal control group,the levels of TC,TG,LDL-C,ALT and AST in model group were increased and content of HDL-C was decreased,with significant difference（P〈0.01）. Compared with model group,the levels of TC,TG,LDL-C,ALT and AST in positive control group and Lin＇s Jiangzhifang high-dose group were decreased and content of HDL-C was increased,with significant difference（P〈0.01）. Contents of TC,TG in Lin＇s Jiangzhifang medium-dose and low-dose groups were decreased and content of HDL-C was increased,activities of AST in Lin＇s Jiangzhifang medium-dose group was deceased,with significant difference（P〈0.01）. The pathological sections showed diffuse cloudy swelling and severe fatty degeneration of the hepatocytes of rats in the model group;mild fatty degeneration of the hepatocytes in Lin＇s Jiangzhifang medium-dose and low-dose groups;hepatocytes were basically normal in Lin＇s Jiangzhifang high-dose group

关 键 词：林氏降脂方 高脂血症 大鼠 血脂 脂肪变性 

分 类 号：R282.6[医药卫生—中药学]