红豆杉单组分多糖的超滤预处理及连续分离工艺  

Ultrafiltration preprocessing and continuous separation process of single-component polysaccharides from Taxus Mairei

作　　者：张菲菲[1,2] 田慧[2] 梁亚非[3] 吴绵斌[1,2] 林建平[1,2] 杨立荣[1,2] 

机构地区：[1]浙江大学生物质化工教育部重点实验室,浙江杭州310027 [2]浙江大学化学工程与生物工程学系,浙江杭州310027 [3]金华市人民医院,浙江金华318100

出　　处：《化工进展》2015年第2期508-513,共6页Chemical Industry and Engineering Progress

基　　金：国家自然科学基金(21376215);浙江省科技计划(2010C33122);国家973计划(2011CB710800);国家863计划(2012AA022302)项目

摘　　要：以红豆杉枝叶部位总多糖为原料,采用超滤方法对原料进行预处理,并对工艺进行优化,最佳优化条件为:截留相对分子质量为5k Da,温度为25℃,压力为20psi(1psi=6894.76k Pa),p H值为6.8。在此条件下,将浓度为10g/L的1L红豆杉多糖溶液超滤至125m L,再添加去离子水至1L,反复超滤4次,得到的红豆杉多糖的截留率和脱色率分别为87.2%和75.6%。进一步以红豆杉单组分多糖间歇色谱分离工艺参数为基础,采用自行设计制作的连续色谱设备,建立了连续离子交换色谱分离红豆杉酸性单组分多糖PST-1的分离工艺,处理量达到每批次100.08L,PST-1纯度在99.0%以上,收率达到50.0%。The total polysaccharides fromTaxus mairei were pretreated by ultrafiltration to remove pigments and the operating condition was optimized as follows：molecular cut-off 5 kDa,temperature 25℃,operating pressure 20psi and pH6.8. Under the aforementioned condition,the 1L solution containing 10g/L total polysaccharides was ultrafiltrated to 125mL first,then deionized water was added into condensed solution to 1L total volume and was repeated 4 times,respectively. The results showed that the retention rate and decolourization ratio of polysaccharides ofTaxus mairei were 87.2% and 75.6%,respectively. Furthermore,the pretreated total polysaccharides ofTaxus mairei were isolated by cation-exchange chromatography using a self-designed continuous chromatography equipment,the processing capacity of which reached 100.08L/d. Based on the operating parameters received from batch chromatography process,99.0% purity single-component polysaccharide（PST-1） was obtained and the average yield of PST-1 reached 50.0%.

关 键 词：红豆杉 多糖 超滤 连续色谱分离 

分 类 号：R284.2[医药卫生—中药学]