猪传染性胃肠炎病毒S基因在昆虫细胞中的表达  

Expression of Spike Gene of Transmissible Gastroenteritis Virus in Insect Cells

作　　者：武岳[1] 周金龙[1] 张超林 张许科[1,2] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]国家兽用药品工程技术研究中心,河南洛阳471003

出　　处：《中国兽药杂志》2015年第3期7-11,共5页Chinese Journal of Veterinary Drug

基　　金：国家"863"计划项目(2011AA10A215)

摘　　要：为获得具有天然活性的猪传染性胃肠炎病毒(TGEV)重组S蛋白,制备针对猪传染性胃肠炎S蛋白A、D抗原位点的单克隆抗体及建立快速抗体检测方法,本研究将TGEV S基因A、D抗原位点经PCR扩增并克隆入p Fast Bac HBM-TOPO载体,经转座、转染后获得重组杆状病毒,并对重组杆状病毒进行间接免疫荧光(IFA)及Western blot分析,结果显示,TGEV S基因A、D抗原位点在杆状病毒中成功表达,其表达产物为TGE诊断试剂的制备、基因工程疫苗的研制奠定了基础。In order to obtain biologically active recombinant S protein of transmissible gastroenteritis virus （ TGEV） , prepare for the monoclonal antibody against A and D antigenic sites of S protein, and establish a rapid antibody detection method, A and D antigenic sites of S gene fragment was amplified by PCR and cloned into pFastBacHBM-TOPO vector, after transposition and transfection, the recombinant baculoviruse was obtained. Then the recombinant baculoviruse was identified by indirect immunofluorescence （ IFA） and western blot, the results showed that TGEV S gene was successfully expressed in baculovirus expression system, which would lay a solid foundation for developing diagnostic reagents and genetic engineering vaccines of TGE.

关 键 词：猪传染性胃肠炎病毒 S基因 杆状病毒表达 

分 类 号：S852.659.6[农业科学—基础兽医学]