正交设计多指标综合评分优化鳖甲微波灭菌工艺  

Optimization of the Microwave Sterilization Technology of Carapax Trionycis by Orthogonal Design with Multi-index Comprehensive Evaluation Method

作　　者：万露[1,2] 闵红燕[1] 肖云芝[3] 张文骞[2] 申宝德[2] 韩晋[2] 袁海龙[2] 

机构地区：[1]江西中医药大学,江西南昌330000 [2]中国人民解放军第三〇二医院,北京100039 [3]成都中医药大学,四川成都611137

出　　处：《亚太传统医药》2015年第6期26-28,共3页Asia-Pacific Traditional Medicine

基　　金：国家新药创制重大专项(2011ZX09201-201-14);军队医疗机构制剂标准提高科研专项(13ZJZ12;13ZJZ12-5)

摘　　要：目的:优选鳖甲的微波灭菌工艺。方法:以药材含湿率、灭菌时间、灭菌功率为考察因素,以灭菌率和鳖甲抗肝纤维化作用(人肝星状细胞LX-2生长抑制率)为评价指标,采用正交设计多指标综合评分法优化微波灭菌鳖甲的工艺参数。结果:最佳微波灭菌工艺参数:灭菌时间为6min,药材含湿率为20%,灭菌功率为4 000W。结论:正交设计多指标综合评分法可用于鳖甲微波灭菌工艺的参数优化,该方法简便可行,且灭菌效率高,对鳖甲抗肝纤维作用无影响。Objective：To optimize the microwave sterilization technology of Carapax Trionycis.Methods：Orthogonal design was carried out to achieve the best microwave sterilization technology of C.Trionycis by the multi-index comprehensive evaluation method with herbs moisture content,sterilization time and microwave power as investigation factors,and sterilization rate and anti-hepatic fibrosis efficacy（the LX-2hepatic stellate cell proliferation inhibition rate）as indexes.Results：The best sterilization technology was as follows：herbs moisture content 20%,sterilization time 6min and microwave power 4 000 W.Conclusion：It is simple and feasible that using orthogonal design with multi-index comprehensive evaluation method to optimize the microwave sterilization technology of C.Trionycis.This method not only has high sterilization efficiency,but also has no effect on C.Trionycis anti-hepatic fibrosis efficacy.

关 键 词：鳖甲 微波灭菌 正交设计 多指标综合评分 

分 类 号：R284[医药卫生—中药学] R954[医药卫生—中医学]