SSR分子标记在糯高粱种质资源遗传多样性分析中的应用  

Genetic diversity analysis of glutinous sorghum germplasm by simple sequence repeat

作　　者：倪先林[1] 赵甘霖[1] 刘天朋[1] 胡炯凌[1] 李元[1] 陈国民[1] 汪小楷[1] 丁国祥[1] 

机构地区：[1]四川省农业科学院水稻高粱研究所,四川泸州646000

出　　处：《江苏农业学报》2015年第1期16-22,共7页Jiangsu Journal of Agricultural Sciences

基　　金：四川省农业科学院青年基金项目(2012QNJJ-023);国家高粱产业技术体系酿酒(糯)高粱新品种选育项目(CARS-06-01-05);四川省财政育种基因工程项目(2011JYGC11-031);四川省农作物育种攻关项目(2011NZ0098-6)

摘　　要：为了阐明糯高粱种质资源之间的亲缘关系,用25对SSR引物评价了29份糯高粱种质资源。结果显示,25对引物共检测出59个等位基因,每对引物检测到2.28个等位基因,平均有效等位基因为1.81个,引物位点的多态信息量变幅为0.17-0.62,平均为0.34。29份糯高粱种质资源的遗传相似系数范围为0.20-0.95,平均为0.60,UPGMA方法将29份糯高粱品种在遗传相似系数0.48处聚为2大类,其中不同地理来源的品种被聚在同一亚类,农艺性状近似的品种被聚在同一亚类。表明糯高粱品种的亲缘关系与地理来源关系不大,其种质资源具有较为丰富的遗传多样性。To evaluate the genetic relationship of 29 glutinous sorghum germplasm,25 pair of simple sequence repeat（ SSR） markers were used. A total of 59 alleles were identified with an average of 2. 28 alleles per locus and the average effective number of alleles（ NE） of 1. 81. The average polymorphism information content（ PIC） varied widely from 0. 17 to 0.62,with an average value of 0. 34. The similarity coefficients of 29 glutinous sorghum accessions ranged from 0. 20 to 0. 95,with an average value of 0. 60. Cluster analysis with UPGMA showed that the varieties could be divided into two groups at the genetic similarity coefficient of 0. 48. The varieties from different geographic origins were clustered into one subclass,and the varieties with similar agronomic traits were classified into one subclass as well,indicating that the geographical origin were irresponsible for the genetic relationship among materials,and the glutinous sorghum germplasm showed abundant genetic diversities.

关 键 词：SSR标记 糯高粱 遗传多样性 

分 类 号：S514.02[农业科学—作物学]