不同中药多糖体外对鸡外周血和脾脏淋巴细胞增殖能力的比较  

Comparative study on chicken peripheral lymphocyte and spleen lymphocyte proliferation by Chinese herbal medicinal polysaccharides in vitro

作　　者：蒋春茂[1] 陈晓兰[1,2] 陆广富[1] 陈兴颖[2] 王德云[2] 胡元亮[2] 耿千丽 

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300 [2]南京农业大学动物医学院,江苏南京2100951

出　　处：《江苏农业学报》2015年第1期106-111,共6页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省自然科学基金面上项目(BK2011537);江苏省农业三新工程项目[SXGC(2013)291];江苏农牧科技职业学院凤凰人才专项(NSFFH1303);江苏省大学生实践创新项目(201412806016Y);学院重点项目(NSFZD1404)

摘　　要：为比较玉竹多糖、桑叶多糖、杜仲多糖、商陆多糖、淫羊藿多糖体外增强免疫活性,采用酶学检测法测定各中药多糖对鸡胚成纤维细胞的安全浓度,根据安全浓度测定结果,选择250.000μg/ml、125.000μg/ml、62.500μg/ml、31.250μg/ml、15.625μg/ml 5个浓度,用MTT法比较5种中药多糖单独和PHA协同刺激对外周血T淋巴细胞增殖指数的影响,以及比较5种中药多糖单独和LPS协同刺激对脾脏B淋巴细胞增殖率的影响。结果显示,玉竹多糖、桑叶多糖、杜仲多糖、商陆多糖、淫羊藿多糖对鸡胚成纤维细胞的安全浓度分别为1 250.0μg/ml、625.0μg/ml、625.0μg/ml、625.0μg/ml、312.5μg/ml。玉竹多糖、桑叶多糖、杜仲多糖和淫羊藿多糖无论是单独还是协同PHA刺激外周血T淋巴细胞,均不同程度地表现出增强T淋巴细胞的活性。桑叶多糖、杜仲多糖和淫羊藿多糖无论是单独还是协同LPS刺激脾脏B淋巴细胞,均不同程度地显示出良好的增强B淋巴细胞的活性。桑叶多糖和杜仲多糖体外增强免疫效果最佳,在62.500μg/ml和125.000μg/ml时,均显示出较强的增强外周血T淋巴细胞和脾脏B淋巴细胞增殖的能力。Polygonatum odoratum polysaccharide（ POPS）,morus polysaccharide（ MPS）,eucommia polysaccharide（ EPS）,pokeweed polysaccharide（ PPS）,and epimedium polysaccharide（ EPPS） to chicken embryonic fibroblast（ CEF） in vitro were determined and showed as follows compared. 1 250. 0 μg / ml,625. 0 μg / ml,625. 0 μg / ml,625. 0 μg / ml,and312. 5 μg / ml,respectively. The effects of above five polysaccharides applied solely or co-applied with phytohaema glutinin（ PHA） on the proliferation of peripheral blood T lymphocytes and the effects of five paly saccharides appiied solely or co-applied with lipopolysaccharide LPS on the proliferation of spleenic B lymphocytes were compared. POPS,MPS,EPSand EPPScould enhance peripheral blood Tlymphocytes activity at different levels either stimulated alone or co-stimulated with PHA. MPS,EPSand EPPScould improve spleenic B lymphocytes activity at different levels either stimulated alone or co-stimulated with LPS. The immune enhancements of MPSand EPSin vitro outperformed others at the concentration of 62. 5 μg / ml and 125 μg / ml.

关 键 词：中药多糖 淋巴细胞增殖 植物血凝素(PHA) 脂多糖(LPS) 

分 类 号：S853.74[农业科学—临床兽医学]