两个红梨品种花色苷合成相关基因及转录因子MYB10表达模式分析  

Analyses of expression patterns of transcription factor MYB10 and anthocyanin synthesis genes in two red skin pear varieties

作　　者：田鹏[1] 苏艳丽[1] 康保珊[1] 魏闻东[1] 

机构地区：[1]中国农业科学院郑州果树研究所,河南郑州450009

出　　处：《江苏农业学报》2015年第1期166-171,共6页Jiangsu Journal of Agricultural Sciences

基　　金：郑州市创新型科技人才队伍建设工程--科技创新团队项目(10CXTD149);河南省重大科技攻关计划项目(092101110400)

摘　　要：为研究不同红色梨品种着色差异的原因,以西洋梨品种红星和砂梨品种满天红为试材,通过荧光定量PCR方法,分析果皮中花色苷合成基因PAL、CHS、CHI、F3H、DFR、ANS、F3GT和转录因子MYB10基因在果实不同发育期的转录特性。结果表明,在红星中除PAL外,花色苷合成基因表达量与果皮花色苷含量变化一致,先升高后降低;在满天红中,PAL和F3GT在果实转色期表达量最高,其他基因表达量随果实发育而下降。转录因子MYB10在红星整个果实发育期表达水平都很低,在满天红转色期呈现峰值,且表达量是红星的50倍以上。红星着色程度可能由多个基因协同作用,而满天红着色可能受关键基因F3GT的影响,MYB10可能通过调控F3GT的表达从而调控满天红的着色。The objective of this study is to reveal the difference of the coloration mechanism between two red skin pear varieties by analyzing the expression of anthocyanin synthesis related genes. Expression of the genes encoding seven enzymes（ PAL,CHS,CHI,F3 H,DFR,ANS,F3GT） and transcription factor MYB10 gene were examined by real-time PCR for fruit skin of two pear varieties,Starkrimson and Mantianhong,at three fruit development stages. In Starkrimson,the expression of the anthocyanin synthesis related genes except for PAL increased and then decreased,in agreement with anthocyanin content changes,during the whole stage of fruit development. However,in Mantianhong,the expressions of PAL and F3 GT were the highest at fruit color turning and other genes decreased gradually along with fruit development.MYB10 gene were lowly expressed during the whole fruit development in Starkrimson,and reached the highest at fruit color turning in Mantianhong, 50 times higher than in Starkrimson. The coloration degree of Starkrimson could be controlled through the coordinated expression of the structural genes. MYB10 might control the coloration in Mantianhong through regulating the expression of key gene F3 GT.

关 键 词：红梨 花色苷 表达 MYB10 荧光定量PCR 

分 类 号：S661.2[农业科学—果树学]