-80℃冻存的自体外周血造血干细胞移植重建造血的观察  

Hematopoietic Reconstitution after Transplantation of Uncontrolled-rate Cryopreservation Autologous Peripheral Blood Hematopoietic Stem Cells Using-80°C Mechanical Freezer

作　　者：刘默[1] 赵瑜[1] 孙敬芬[1] 赵伟[1] 王莉莉[1] 于力[1] 

机构地区：[1]中国人民解放军总医院血液科,北京100853

出　　处：《中国实验血液学杂志》2015年第1期166-172,共7页Journal of Experimental Hematology

摘　　要：目的:本研究旨在明确-80℃非程控降温对于保存外周血造血干细胞的效果。方法:观察104例自体移植的病例,外周血造血干细胞采用-80℃非程控降温,采用5%的二甲亚砜(DMSO)、20%人血白蛋白(HSA)以及羟乙基淀粉40(HES)作为冻存保护液,中位冻存时间为30.5 d,观察冻存前后细胞活性、CD34+细胞、单个核细胞(mononuclear cells,MNCs)以及植入情况。结果:本冻存方式可以在短时间内很好的保存外周血造血干细胞。104例患者除2例发生移植相关死亡外,余患者均顺利植入,白细胞中位植入时间为12 d,血小板中位植入时间为14d,复苏后细胞活性为94%,CD34+细胞回收率为86%,单个核细胞回收率为80.3%,性别,化疗周期和放疗对于植入时间均无明显影响,白细胞的植入与CD34+细胞的输注量呈线性关系。结论:本研究的冻存方式安全、有效,完全可以满足临床需求。这种节约资金、人力的干细胞保存方式可以被广泛地应用和推广。Objective：This study was to identify the efficacy of -80℃ cryopreservated peripheral blood hematopoietic stem cell （PBHSC） transplantation for hematopoietic reanstitution in patients. Methods： The efficacy of 104 patients underwent autologous peripheral blood hematopoietic stem cell transplantation using uncontrolled-rate freezing and storage at - 80℃ was evaluated. Results： This cryopreservation method could effectively cryopreserve peripheral blood stem ceils. Out of 104 patients only 2 patients died, other patients got hematologic reconstition satisfactorily, the median engrafement times of neutrophils and platelet were 12 and 14 days respectively, the activity of cells after rehabilitation was 94%, the mean recovery rates of CD34 ＋ cells and mononuclear cells （MNC） were 86% and 80.3% respectively. There were no significant influences on engrafement time in sex, chemotherapy circles and radiotherapy. The engrafement of leukocytes associated with amount of CD34 ＋ cells. Conclusion： This simple uncontrolled - rate freezing PBHSC at -80~C is safe, effective and economic, and can meet clinical needs. As compared with the classical cryopreservation, there were no significant differences in hematopoietic reconstitution. Therefore, this method worth to popularize and apply in clinic.

关 键 词：-80℃冻存 外周血造血干细胞 自体移植 

分 类 号：R452.7[医药卫生—治疗学] R318.52[医药卫生—临床医学]