小鼠精原细胞的分离纯化  

Isolation and Purification of Mouse Spermatogonia

作　　者：李玉华[1,2] 段斐[2] 周小羽[2] 李扬[1] 李润生[2] 

机构地区：[1]吉林大学白求恩医学院病理生理学教研室,长春130021 [2]上海市计划生育科学研究所,上海200032

出　　处：《实验动物与比较医学》2015年第1期42-45,共4页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金资助项目（编号81270760）

摘　　要：目的建立一种高效、简便的分离纯化小鼠精原细胞的方法。方法利用胶原酶IV／脱氧核糖核酸酶I（DNaseI）／分散酶（dispase）组合酶消化法和差异性贴壁原理，从新生小鼠睾丸中分离、纯化精原细胞。经细胞形态学观察、视黄酸激活基因8（Stra8）免疫化学鉴定精原细胞并进行纯度分析。结果分离到的细胞经Stra8检测呈阳性，精原细胞纯度可达86％。结论该方法可高效地分离和纯化小鼠精原细胞。Objective To establish a simple and efficient method for separation and purification of mouse spermatogonia. Methods Spermatogonia were isolated and purified from neonatal mouse testis using collagenase IV/DNase I/dispase combination enzymatic digestion and differential velocity of cell adherence method. Isolated spermatogonia were analyzed by morphology and stra8 immunochemistry. Results The isolated spermatogonia were stra8 positive and cell purity was as high as 86%. Condusion This method is efficient for separation and purification of mouse spermatogonia

关 键 词：小鼠 精原细胞 分离 纯化 

分 类 号：Q95-33[生物学—动物学]