三种大鼠骨质疏松模型的比较研究  

Comparative Study on Three Kinds of Osteoporosis Model in Rats

作　　者：冯佳 向阳 夏燕 陈安平 杨年安 龚书识 袁林 

机构地区：[1]风湿性疾病发生与干预湖北省重点实验室,恩施445000 [2]湖北民族学院附属民大医院,恩施445000

出　　处：《实验动物与比较医学》2015年第1期52-55,共4页Laboratory Animal and Comparative Medicine

基　　金：湖北民族学院校园项目（MY20118008）;湖北民族学院民大医院院内课题（2013.6）,湖北医学领军人才培养工程专项经费资助

摘　　要：目的观察和比较不同造模方法对大鼠骨质疏松模型特点的影响。方法选用6月龄的雌性sD大鼠40只，分为正常组、去势组、糖皮质激素组和维甲酸组。造模6周后，比较各组大鼠血清钙（Ca）、磷（P）、碱性磷酸酶（ALP）、骨密度和骨组织形态计量学参数的变化。结果与正常组相比，去势组及维甲酸组大鼠血清Ca降低，P、ALP增高，股骨骨密度、骨小梁厚度、数量均明显减少，骨小梁间距增大；糖皮质激素组与正常组相比，各参数无显著差异。结论去势骨质疏松模型造模成功。短期间断使用糖皮质激素对大鼠松质骨的影响不明显；大剂量维甲酸能使大鼠出现骨量减少，骨微结构发生改变，但其毒副作用较大。Objective To observe and compare the effects of different modeling methods of osteoporosis on rats. Methods Female SD rats at age of six months were divided into normal group, ovariectomized group, glucocorticoid group, and retinoic acid group. After 6 weeks, the changes of serum calcium, phosphorus, alkaline phosphatase and bone density, bone histomorphometry parameters were compared. Results Compared with normal group, the serum Ca in ovariectomized rats was decreased, serum P and ALP were increased, bone mineral density, trabecular thickness and trabecular number were decreased, and trabecular spacing was increased.The Glucocorticoid group compared with normal group, there was no significant difference in each parameters. Compared with the normal group, retinoic acid rat＇s serum Ca was decreased, serum P and ALP were increased,bone mineral density, trabecular thickness and trabecular number were decreased, and trabecular spacing was increased. Conclusion The osteoporotic model was successful.With the short-term intermittent use of glucocorticoid, effects on the cancellous bone in rats may not be obvious, large dose of retinoic acid can reduce bone mass of rats in short-term and chan^e the bone micro structural, but it had obvious side effects.

关 键 词：骨质疏松(OP) 去势(OVX) 糖皮质激素(GC) 维甲酸(RA) 大鼠模型 

分 类 号：R580[医药卫生—内分泌] R-332[医药卫生—内科学]